Mutational analysis of bovine papillomavirus E6 gene.
The bovine papillomavirus E6 gene can independently transform mouse C127 cells. To characterize E6 in greater detail, we created 16 site-directed mutations in E6, including substitution mutations in the cysteine codons of the four Cys-X-X-Cys motifs that are conserved in all papillomavirus E6 proteins. Proteins mutated in six of the seven cysteines tested, as well as those lacking the nonconserved C-terminus, were stable in transfected cells but were unable to induce morphological transformation, indicating that these amino acids play an important role in the function of E6.
15 references, click to expand
-
Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.J Mol Appl Genet. 1982;1(4):327-41 PMID: 6286831
-
Zinc fingers: gilt by association.Cell. 1988 Jan 15;52(1):1-3 PMID: 3125980
-
Rapid and efficient site-specific mutagenesis without phenotypic selection.Proc Natl Acad Sci U S A. 1985 Jan;82(2):488-92 PMID: 3881765
-
Identification of the protein encoded by the E6 transforming gene of bovine papillomavirus.Science. 1985 Oct 25;230(4724):442-5 PMID: 2996134
-
Dissociation of transforming and trans-activation functions for bovine papillomavirus type 1.Nature. 1985 Dec 12-18;318(6046):575-7 PMID: 2999614
-
E5 open reading frame of bovine papillomavirus type 1 encodes a transforming gene.J Virol. 1986 Jan;57(1):1-6 PMID: 3001335
-
Transcription of human papillomavirus type 16 early genes in a cervical cancer and a cancer-derived cell line and identification of the E7 protein.Proc Natl Acad Sci U S A. 1986 Jul;83(13):4680-4 PMID: 3014503
-
Repression of bovine papilloma virus replication is mediated by a virally encoded trans-acting factor.Cell. 1986 Aug 29;46(5):753-62 PMID: 3017567
-
Structural and transcriptional analysis of human papillomavirus type 16 sequences in cervical carcinoma cell lines.J Virol. 1987 Apr;61(4):962-71 PMID: 3029430
-
Metal-dependent folding of a single zinc finger from transcription factor IIIA.Proc Natl Acad Sci U S A. 1987 Jul;84(14):4841-5 PMID: 3474629
-
Nucleotide sequence and comparative analysis of the human papillomavirus type 18 genome. Phylogeny of papillomaviruses and repeated structure of the E6 and E7 gene products.J Mol Biol. 1987 Feb 20;193(4):599-608 PMID: 3039146
-
Transcriptional differences of the human papillomavirus type 16 genome between precancerous lesions and invasive carcinomas.J Virol. 1988 Mar;62(3):1022-7 PMID: 2828651
-
Papillomavirus polypeptides E6 and E7 are zinc-binding proteins.J Virol. 1989 Mar;63(3):1404-7 PMID: 2536841
-
The E7 open reading frame of human papillomavirus type 16 encodes a transforming gene.Oncogene Res. 1988 Sep;3(2):167-75 PMID: 2852339
-
Identification of a second transforming region in bovine papillomavirus DNA.Proc Natl Acad Sci U S A. 1984 Dec;81(24):7880-4 PMID: 6096867
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: product@genelibs.com
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
