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PMID: 2159142 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Method to identify genomic targets of DNA binding proteins.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 9 ·1990-05-00 ·Pages 3274-8

Sompayrac L, Danna KJ

Abstract

We have devised a cyclical immunoprecipitation protocol that can be used to identify and clone a specific DNA sequence that is recognized by a DNA binding protein, even if that sequence is present in only one copy in the genome of a mammal. As an example, we have used this procedure to purify mouse genomic sequences to which the simian virus 40 tumor (T) antigen binds.

MeSH Terms
Adenoviruses, Human/genetics,immunology Animals Antigens, Polyomavirus Transforming Binding Sites Cell Line Cells, Cultured DNA/metabolism DNA, Viral/metabolism DNA-Binding Proteins/isolation & purification,metabolism Genomic Library Humans Mice Plasmids Simian virus 40/genetics,immunology Staphylococcus aureus/genetics
Chemicals
Antigens, Polyomavirus Transforming DNA, Viral DNA-Binding Proteins DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sompayrac L
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80309-0347.
Danna K J
References (19)
19 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.9.3274
Published
1990-05-00
Pages
3274-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC53882
Subset
IM
Grants
NCI NIH HHS · CA-34072 · United States
External Links
PubMed source DOI: 10.1073/pnas.87.9.3274 PMC Full Text View journal details
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