Home LiteratureArticle Details
PMID: 3697081 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Application of an immunoprecipitation procedure to the study of SV40 tumor antigen interaction with mouse genomic DNA sequences.

Nucleic acids research ·Vol. 15 ·No. 23 ·1987-12-10 ·Pages 9741-59

Pollwein P, Wagner S, Knippers R

Abstract

Simian Virus 40 (SV40) large T antigen is a DNA binding protein with high affinity for segments of the viral genome. To find out whether T antigen also binds to sequences of genomic cellular DNA we mixed T antigen and SAU 3 A restricted mouse DNA under stringent DNA binding conditions. Resulting protein-DNA complexes were immunoprecipitated using T antigen specific monoclonal or polyclonal antibodies. The DNA fragments in the immunoprecipitates were cloned in plasmid vectors. Four plasmid clones were selected for a detailed investigation of the inserted mouse DNA fragments. Nucleotide sequencing and DNase I footprint experiments showed that T antigen binds to sites in these fragments consisting of two tandemly oriented G(A)AGGC pentamers separated by AT rich spacers of different lengths. The cellular binding sites are very similar in their architecture to the SV40-DNA binding site I. The isolated cellular DNA fragments with T antigen binding sites occur only once or a few times in the mouse genome. Our data help to further define the structure of T antigen's DNA binding sites. The genetic functions of the isolated cellular DNA elements are not known.

MeSH Terms
Animals Antigens, Polyomavirus Transforming/analysis Base Sequence Cell Line, Transformed DNA/genetics,metabolism DNA-Binding Proteins/analysis Mice Molecular Sequence Data Nucleic Acid Hybridization Precipitin Tests/methods
Chemicals
Antigens, Polyomavirus Transforming DNA-Binding Proteins DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Pollwein P
Fakultät für Biologie, Universität Konstanz, FRG.
Wagner S
Knippers R
References (42)
42 references, click to expand
  1. Virogenic properties of bromodeoxyuridine-sensitive and bromodeoxyuridine-resistant simian virus 40-transformed mouse kidney cells.
    J Virol. 1967 Oct;1(5):968-79 PMID: 4316241
  2. Genetic and biochemical analysis of transformation-competent, replication-defective simian virus 40 large T antigen mutants.
    J Virol. 1985 Jan;53(1):120-7 PMID: 2981330
  3. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  4. DNAse footprinting: a simple method for the detection of protein-DNA binding specificity.
    Nucleic Acids Res. 1978 Sep;5(9):3157-70 PMID: 212715
  5. Nucleotide sequences of integrated Moloney sarcoma provirus long terminal repeats and their host and viral junctions.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3937-41 PMID: 6254003
  6. Regulation of simian virus 40 early transcription in vitro by a purified tumor antigen.
    Proc Natl Acad Sci U S A. 1980 Oct;77(10):5706-10 PMID: 6255460
  7. Monoclonal antibodies against simian virus 40 tumor antigens: analysis of antigenic binding sites, using adenovirus type 2-simian virus 40 hybrid viruses.
    J Virol. 1981 Jan;37(1):478-82 PMID: 6163870
  8. Binding of a simian virus 40 T antigen-related protein to DNA.
    J Mol Biol. 1981 Jan 25;145(3):471-88 PMID: 6267291
  9. Alternative interactions of the SV40 A protein with DNA.
    Virology. 1981 Nov;115(1):75-87 PMID: 6270902
  10. Regulatory mutants of simian virus 40. Effect of mutations at a T antigen binding site on DNA replication and expression of viral genes.
    J Mol Biol. 1982 Apr 15;156(3):531-48 PMID: 6288959
  11. Sequence-specific binding of simian virus 40 A protein to nonorigin and cellular DNA.
    Mol Cell Biol. 1984 Dec;4(12):2631-8 PMID: 6570189
  12. A large-tumor-antigen-specific monoclonal antibody inhibits DNA replication of simian virus 40 minichromosomes in an in vitro elongation system.
    J Virol. 1985 May;54(2):473-82 PMID: 2985809
  13. Expression of enhanced levels of small RNA polymerase III transcripts encoded by the B2 repeats in simian virus 40-transformed mouse cells.
    Nature. 1985 Apr 11-17;314(6011):553-6 PMID: 2581137
  14. Differential ability of a T-antigen transport-defective mutant of simian virus 40 to transform primary and established rodent cells.
    Mol Cell Biol. 1985 May;5(5):1043-50 PMID: 2987672
  15. Monomers through trimers of large tumor antigen bind in region I and monomers through tetramers bind in region II of simian virus 40 origin of replication DNA as stable structures in solution.
    Proc Natl Acad Sci U S A. 1985 Jun;82(11):3626-30 PMID: 2987937
  16. Seventeen base pairs of region I encode a novel tripartite binding signal for SV40 T antigen.
    Cell. 1985 Sep;42(2):539-48 PMID: 2992803
  17. Identification and biochemical analysis of DNA replication-defective large T antigens from SV40-transformed cells.
    Virology. 1985 Oct 30;146(2):188-202 PMID: 2996217
  18. Cellular targets for SV40 large T-antigen.
    Proc R Soc Lond B Biol Sci. 1985 Oct 22;226(1242):25-42 PMID: 2415984
  19. A new sensitive target-bound DNA binding assay for SV40 large T antigen.
    Virology. 1986 Jan 15;148(1):159-67 PMID: 3002028
  20. An altered DNA conformation in origin region I is a determinant for the binding of SV40 large T antigen.
    Cell. 1986 Mar 14;44(5):719-25 PMID: 3004744
  21. Replication of a plasmid bearing a human Alu-family repeat in monkey COS-7 cells.
    Proc Natl Acad Sci U S A. 1986 Jul;83(13):4660-4 PMID: 3014501
  22. Replication and supercoiling of simian virus 40 DNA in cell extracts from human cells.
    Mol Cell Biol. 1985 Aug;5(8):2051-60 PMID: 3018548
  23. DNA helicase activity of SV40 large tumor antigen.
    EMBO J. 1986 Aug;5(8):1939-44 PMID: 3019672
  24. Functional organization of the simian virus 40 origin of DNA replication.
    Mol Cell Biol. 1986 Apr;6(4):1117-28 PMID: 3023870
  25. trans Activation of the simian virus 40 enhancer.
    Mol Cell Biol. 1986 Apr;6(4):1283-95 PMID: 3023880
  26. Domain structure of the simian virus 40 core origin of replication.
    Mol Cell Biol. 1986 May;6(5):1663-70 PMID: 3023900
  27. A second domain of simian virus 40 T antigen in which mutations can alter the cellular localization of the antigen.
    Mol Cell Biol. 1986 Jun;6(6):2207-12 PMID: 3023922
  28. Binding of polyomavirus large T antigen to the human hsp70 promoter is not required for trans activation.
    Mol Cell Biol. 1986 Sep;6(9):3180-90 PMID: 3023966
  29. Monoclonal antibodies as probes for a function of large T antigen during the elongation process of simian virus 40 DNA replication.
    J Virol. 1987 Feb;61(2):411-8 PMID: 3027371
  30. The T-antigen-binding domain of the simian virus 40 core origin of replication.
    J Virol. 1987 Jul;61(7):2143-9 PMID: 3035215
  31. SV40 large tumor antigen can regulate some cellular transcripts in a positive fashion.
    Cell. 1982 Sep;30(2):481-90 PMID: 6291771
  32. Subclasses of simian-virus-40 large tumor antigen. Partial purification and DNA-binding properties of two subclasses of tumor antigen from productively infected cells.
    Eur J Biochem. 1982 Nov;128(1):53-62 PMID: 6293828
  33. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
    Gene. 1982 Oct;19(3):259-68 PMID: 6295879
  34. Topography of simian virus 40 A protein-DNA complexes: arrangement of protein bound to the origin of replication.
    J Virol. 1983 Apr;46(1):151-61 PMID: 6298452
  35. Mutational analysis of simian virus 40 T antigen: stimulation of cellular DNA synthesis and activation of rRNA genes by mutants with deletions in the T-antigen gene.
    Mol Cell Biol. 1983 Feb;3(2):214-9 PMID: 6300657
  36. Biochemical activities of T-antigen proteins encoded by simian virus 40 A gene deletion mutants.
    Mol Cell Biol. 1983 Feb;3(2):220-8 PMID: 6300658
  37. Activation of mouse genes in transformed cells.
    Cell. 1983 Sep;34(2):557-67 PMID: 6311432
  38. Binding of simian virus 40 a protein to DNA with deletions at the origin of replication.
    J Virol. 1984 Jan;49(1):9-13 PMID: 6317897
  39. Essential contact residues within SV40 large T antigen binding sites I and II identified by alkylation-interference.
    Cell. 1984 Jan;36(1):155-62 PMID: 6319005
  40. Characterization of DNA sequences through which cadmium and glucocorticoid hormones induce human metallothionein-IIA gene.
    Nature. 1984 Apr 5-11;308(5959):513-9 PMID: 6323998
  41. Simian virus 40 DNA replication in vitro.
    Proc Natl Acad Sci U S A. 1984 Nov;81(22):6973-7 PMID: 6095264
  42. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.23.9741
Published
1987-12-10
Pages
9741-59
Language
English
Region
England
NLM ID
0411011
PMCID
PMC306528
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/15.23.9741 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2