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PMID: 2157625 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Comparison of thermosensitive alleles of the CDC25 gene involved in the cAMP metabolism of Saccharomyces cerevisiae.

Genetics ·Vol. 124 ·No. 4 ·1990-04-00 ·Pages 797-806

Petitjean A, Hilger F, Tatchell K

Abstract

The CDC25 gene from Saccharomyces cerevisiae is an essential component of the RAS-adenylate cyclase pathway. Genetic and biochemical evidence has led to the proposal that the gene product may act upstream of RAS, possibly as a guanine nucleotide exchange factor. We report here the cloning, sequencing and characterization of four mutations in the CDC25 gene. All four are missense mutations which reside within the carboxy-terminal quarter of the single open reading frame found within the gene. Three of the four are missense mutations in the same amino acid codon. A search of protein data bases reveals that the carboxy terminus of the putative CDC25 gene product is similar to that of LTE1, a gene required for growth at low temperature and SCD25, a suppressor of cdc25. Taken together these data indicate that the carboxy terminus of CDC25 plays a critical role in the function of the CDC25 gene product and that other proteins, such as LTE1 or SCD25, may have related activities.

MeSH Terms
Adenylyl Cyclases/genetics,metabolism Alleles Amino Acid Sequence Base Sequence Cell Cycle Proteins Cloning, Molecular Cyclic AMP/metabolism Fungal Proteins/genetics Genes, Fungal Genes, ras Molecular Sequence Data Mutation Phenotype Restriction Mapping Saccharomyces cerevisiae/genetics,metabolism Suppression, Genetic Temperature ras-GRF1
Chemicals
Cell Cycle Proteins Fungal Proteins ras-GRF1 Cyclic AMP Adenylyl Cyclases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Petitjean A
Faculté des Sciences Agronomiques, Laboratoire de Microbiologie, Gambloux, Belgium.
Hilger F
Tatchell K
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45 references, click to expand
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/124.4.797
Published
1990-04-00
Pages
797-806
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1203972
Subset
IM
Grants
NCI NIH HHS · CA37702 · United States
External Links
PubMed source DOI: 10.1093/genetics/124.4.797 PMC Full Text View journal details
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