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PMID: 2155782 Published · ppublish English Journal Article

A major thyroid hormone response element in the third intron of the rat growth hormone gene.

The EMBO journal ·Vol. 9 ·No. 3 ·1990-03-00 ·Pages 887-96

Sap J, de Magistris L, Stunnenberg H, Vennström B

Abstract

The rat growth hormone (RGH) gene constitutes a well-documented model system for the direct regulation of transcription by thyroid hormones. In order to analyse its interaction with sequences in the RGH gene, we have overproduced the thyroid hormone receptor-alpha (c-erbA) protein using a vaccinia virus expression system. The expressed protein bound T3 and DNA-cellulose with expected affinities, and the major binding site for the receptor protein was found to be located in the third intron of the RGH gene. This site displayed significantly higher affinity for the receptor protein than a previously described thyroid hormone response element (TRE) in the promoter of this gene, and also conferred stronger hormone responsiveness in vivo to a heterologous promoter. The data suggest that this novel TRE plays a major role in the regulation of rat growth hormone gene expression by thyroid hormones.

MeSH Terms
Animals Base Sequence Binding Sites Cell Line Cloning, Molecular Deoxyribonuclease I Gene Expression Regulation/drug effects Genes Growth Hormone/genetics HeLa Cells/metabolism Humans Introns Molecular Sequence Data Mutation Plasmids Promoter Regions, Genetic Protein-Tyrosine Kinases/metabolism Proto-Oncogene Proteins/metabolism Rats Receptors, Thyroid Hormone Restriction Mapping Transcription, Genetic/drug effects Transfection Triiodothyronine/pharmacology
Chemicals
Proto-Oncogene Proteins Receptors, Thyroid Hormone Triiodothyronine Growth Hormone Protein-Tyrosine Kinases Deoxyribonuclease I
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sap J
EMBL, Differentiation and Gene Expression Programmes, Heidelberg, FRG.
de Magistris L
Stunnenberg H
Vennström B
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38 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-03-00
Pages
887-96
Language
English
Region
England
NLM ID
8208664
PMCID
PMC551749
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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