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PMID: 2153396 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

C-erbB2 mRNA expression in human breast tumours: comparison with c-erbB2 DNA amplification and correlation with prognosis.

British journal of cancer ·Vol. 61 ·No. 1 ·1990-01-00 ·Pages 39-45

Parkes HC, Lillycrop K, Howell A, Craig RK

Abstract

In this study, we have investigated the expression of the proto-oncogene c-erbB2 in a total of 70 human primary breast tumours. In agreement with other workers, we observed c-erbB2 gene amplification in 17.5% of the tumours studied. In addition, we carried out a comprehensive analysis of c-erbB2 mRNA expression in the tumours using RNase mapping and in situ hybridisation techniques. Our results indicated a more frequent (30%) overexpression of c-erbB2 mRNA, which was associated only with breast carcinomas of a ductal origin. Furthermore, analysis of the c-erbB2 mRNA gene locus in the same tumours demonstrated that enhanced c-erbB2 expression could occur in the presence or absence of gene amplification, suggesting that additional molecular mechanisms may result in overexpression of c-erbB2 mRNA in human mammary tumours. In situ hybridisation showed that elevated levels of c-erbB2 mRNA were specific to malignant cells within the breast tumour. Analysis of the association between c-erbB2 mRNA overexpression and clinicopathological factors revealed a significant correlation with poor tumour grade, but not with steroid receptor status or patient menopausal status. No significant correlation was observed between overexpression of c-erbB2 mRNA and early disease recurrence in our group of patients, although there was a definite trend towards poorer prognosis.

MeSH Terms
Adult Aged Aged, 80 and over Breast Neoplasms/chemistry,genetics Carcinoma, Intraductal, Noninfiltrating/chemistry,genetics DNA, Neoplasm/analysis ErbB Receptors Gene Amplification Humans Middle Aged Prognosis Proto-Oncogene Mas Proto-Oncogene Proteins/genetics RNA, Messenger/analysis RNA, Neoplasm/analysis
Chemicals
DNA, Neoplasm MAS1 protein, human Proto-Oncogene Mas Proto-Oncogene Proteins RNA, Messenger RNA, Neoplasm ErbB Receptors
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Parkes H C
Department of Biochemistry, University College, London, UK.
Lillycrop K
Howell A
Craig R K
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Article Info
Journal
British journal of cancer
Abbr.
Br J Cancer
ISSN
0007-0920
DOI
10.1038/bjc.1990.9
Published
1990-01-00
Pages
39-45
Language
English
Region
England
NLM ID
0370635
PMCID
PMC1971341
Subset
IM
External Links
PubMed source DOI: 10.1038/bjc.1990.9 PMC Full Text View journal details
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