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PMID: 3224077 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

In situ hybridisation and S1 mapping show that the presence of infiltrating plasma cells is associated with poor prognosis in breast cancer.

British journal of cancer ·Vol. 58 ·No. 6 ·1988-12-00 ·Pages 715-22

Parkes H, Collis P, Baildam A, Ralphs D, Lyons B, Howell A, Craig R

Abstract

In order to identify potential markers of prognosis in breast cancer, representative cDNA libraries were constructed using RNA isolated from primary breast tumour tissue associated with good and poor prognosis. Cross-screening of these libraries repeatedly identified cloned mRNA species associated with the immune system, in particular B-cells, in libraries derived from tumours of poor prognosis. We have used one of these a kappa IV light chain cDNA probe, in two complementary studies to investigate the relationship between immunoglobin gene expression and prognosis. The results obtained using a combination of S1 mapping, RNA blotting and in situ hybridisation demonstrate that the presence of plasma cells, as defined by infiltrating cells which express high levels of immunoglobulin kappa-chain mRNA, is associated with a poor prognosis.

MeSH Terms
Adult Aged Biomarkers, Tumor Breast Neoplasms/genetics,immunology DNA Probes DNA, Neoplasm/analysis Female Genes, Immunoglobulin Humans Middle Aged Nucleic Acid Hybridization Plasma Cells/pathology Poly A/analysis Prognosis RNA, Messenger/biosynthesis RNA, Neoplasm/analysis Receptors, Antigen, T-Cell/immunology Restriction Mapping
Chemicals
Biomarkers, Tumor DNA Probes DNA, Neoplasm RNA, Messenger RNA, Neoplasm Receptors, Antigen, T-Cell Poly A
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Parkes H
Cancer Research Campaign Tumour Endocrinology Group, Medical Molecular Biology Unit, University College and Middlesex School of Medicine, London, UK.
Collis P
Baildam A
Ralphs D
Lyons B
Howell A
Craig R
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Article Info
Journal
British journal of cancer
Abbr.
Br J Cancer
ISSN
0007-0920
DOI
10.1038/bjc.1988.296
Published
1988-12-00
Pages
715-22
Language
English
Region
England
NLM ID
0370635
PMCID
PMC2246871
Subset
IM
External Links
PubMed source DOI: 10.1038/bjc.1988.296 PMC Full Text View journal details
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