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PMID: 21515767 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification and characterization of CdgB, a diguanylate cyclase involved in developmental processes in Streptomyces coelicolor.

Journal of bacteriology ·Vol. 193 ·No. 12 ·2011-06-00 ·Pages 3100-8

Tran NT, Den Hengst CD, Gomez-Escribano JP, Buttner MJ

Abstract

We describe the identification and functional characterization of cdgB (SCO4281), a recently discovered target of BldD, a key regulator of morphological differentiation and antibiotic production in the mycelial bacteria of the genus Streptomyces. cdgB (cyclic dimeric GMP [c-di-GMP] B) encodes a GGDEF-containing protein that has diguanylate cyclase activity in vitro. Consistent with this enzymatic activity, heterologous expression of cdgB in Escherichia coli resulted in increased production of extracellular matrix in colonies and enhanced surface attachment of cells in standing liquid cultures. In Streptomyces coelicolor, both overexpression and deletion of cdgB inhibited aerial-mycelium formation, and overexpression also inhibited production of the antibiotic actinorhodin, implicating c-di-GMP in the regulation of developmental processes in Streptomyces.

MeSH Terms
Anthraquinones/metabolism Bacterial Proteins/genetics,metabolism DNA-Binding Proteins/genetics,metabolism Escherichia coli Proteins/genetics,metabolism Gene Expression Regulation, Bacterial/physiology Gene Expression Regulation, Enzymologic/physiology Phosphorus-Oxygen Lyases/genetics,metabolism Streptomyces coelicolor/enzymology,metabolism Transcription Factors/genetics,metabolism
Chemicals
Anthraquinones Bacterial Proteins BldD protein, Streptomyces coelicolor DNA-Binding Proteins Escherichia coli Proteins Transcription Factors Phosphorus-Oxygen Lyases diguanylate cyclase actinorhodin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Tran Ngat T
Department of Molecular Microbiology, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK.
Den Hengst Chris D
Gomez-Escribano Juan Pablo
Buttner Mark J
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
1098-5530
DOI
10.1128/JB.01460-10
Published
2011-06-00
Epub
2011-00-22
Pages
3100-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC3133206
Subset
IM
Grants
Biotechnology and Biological Sciences Research Council · BBS/E/J/00000015 · United Kingdom
Biotechnology and Biological Sciences Research Council · BB/H006125/1 · United Kingdom
External Links
PubMed source DOI: 10.1128/JB.01460-10 PMC Full Text View journal details
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