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PMID: 12923110 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Integration site for Streptomyces phage phiBT1 and development of site-specific integrating vectors.

Journal of bacteriology ·Vol. 185 ·No. 17 ·2003-09-00 ·Pages 5320-3

Gregory MA, Till R, Smith MC

Abstract

Despite extensive similarities between the genomes of the Streptomyces temperate phages phiC31 and phiBT1, the attP-int loci are poorly conserved. Here we demonstrate that phiBT1 integrates into a different attachment site than phiC31. phiBT1 attB lies within SCO4848 encoding a 79-amino-acid putative integral membrane protein. Integration vectors based on phiBT1 integrase were shown to have a broad host range and are fully compatible with those based on the phiC31 attP-int locus.

MeSH Terms
Amino Acid Sequence Attachment Sites, Microbiological/genetics Base Sequence Genetic Vectors Membrane Proteins/genetics Sequence Analysis, DNA Siphoviridae/genetics Streptomyces/virology Virus Integration
Chemicals
Membrane Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gregory Matthew A
Institute of Genetics, University of Nottingham, Queens Medical Centre, Nottingham NG7 2UH, United Kingdom.
Till Rob
Smith Margaret C M
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11 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.185.17.5320-5323.2003
Published
2003-09-00
Pages
5320-3
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC180994
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.185.17.5320-5323.2003 PMC Full Text View journal details
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