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PMID: 2147512 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Differential regulation of human immunodeficiency viruses (HIVs): a specific regulatory element in HIV-2 responds to stimulation of the T-cell antigen receptor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 23 ·1990-12-00 ·Pages 9098-102

Markovitz DM, Hannibal M, Perez VL, Gauntt C, Folks TM, Nabel GJ

Abstract

The human immunodeficiency viruses (HIVs) types 1 and 2 have similar genetic organization but differ significantly in nucleic acid sequence. Although infection by either agent leads to symptoms of immunodeficiency, recent studies suggest potential differences in the time course and severity of these diseases. In this report, the transcriptional regulation and induction of these retroviruses were analyzed. We report that the regulation of HIV-2 differs from that of HIV-1: a distinct T-cell activation pathway, triggering of the CD3 component of the T-cell receptor complex, stimulates HIV-2 but not HIV-1 gene expression. The response to T-cell receptor stimulation in HIV-2 is mediated partly by an upstream regulatory element, termed CD3R, which is recognized by a sequence-specific DNA binding protein, NF-CD3R. Jurkat T leukemia cell lines containing HIV-2 provirus also showed increased viral replication after stimulation of the T-cell receptor complex, in contrast to HIV-1. These findings suggest that transcriptional regulation and induction of HIV-2 differ from HIV-1 and raise the possibility that different cofactors contribute to the activation of HIV-1- and HIV-2-associated AIDS.

MeSH Terms
Antigens, CD/immunology Antigens, Differentiation, T-Lymphocyte/immunology Base Sequence CD3 Complex Cell Line Chromosome Deletion Enhancer Elements, Genetic/drug effects HIV-1/genetics HIV-2/genetics Humans Molecular Sequence Data Mutagenesis, Site-Directed Plasmids Receptors, Antigen, T-Cell/immunology,metabolism Regulatory Sequences, Nucleic Acid Restriction Mapping T-Lymphocytes/immunology Tetradecanoylphorbol Acetate/pharmacology Transfection
Chemicals
Antigens, CD Antigens, Differentiation, T-Lymphocyte CD3 Complex Receptors, Antigen, T-Cell Tetradecanoylphorbol Acetate
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Markovitz D M
Howard Hughes Medical Institute, Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor 48109-0650.
Hannibal M
Perez V L
Gauntt C
Folks T M
Nabel G J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.23.9098
Published
1990-12-00
Pages
9098-102
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC55111
Subset
IM
Grants
NIAID NIH HHS · AI26865 · United States
NIAID NIH HHS · AI29179 · United States
External Links
PubMed source DOI: 10.1073/pnas.87.23.9098 PMC Full Text View journal details
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