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PMID: 2144276 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Nitrate regulation of anaerobic respiratory gene expression in narX deletion mutants of Escherichia coli K-12.

Journal of bacteriology ·Vol. 172 ·No. 9 ·1990-09-00 ·Pages 5020-9

Egan SM, Stewart V

Abstract

Previous studies have shown that narL+ is required for nitrate regulation of anaerobic respiratory enzyme synthesis, including formate dehydrogenase-N, nitrate reductase, and fumarate reductase. Insertions in the closely linked narX gene decrease, but do not abolish, nitrate regulation of anaerobic enzyme synthesis. Analysis of sequence similarities suggests that NarX and NarL comprise a two-component regulatory pair. We constructed lacZ operon and gene fusions to investigate the operon structure of narXL. We found evidence for a complex operon with at least two promoters; PXL-narX-PL-narL. We also investigated the role of NarX in nitrate regulation of anaerobic respiratory enzyme synthesis by constructing nonpolar loss of function narX alleles. These deletions were studied on narL+ lambda specialized transducing bacteriophage. The narX deletions had no effect on nitrate regulation in delta (narXL) strains. This finding suggest that the subtle effects of previously studied narX insertions are due to decreased expression of narL and that narX+ is not essential for normal nitrate regulation. The role of NarX in nitrate regulation remains to be determined.

MeSH Terms
Alleles Anaerobiosis Bacteriophage lambda/genetics Base Sequence Chromosome Deletion Cloning, Molecular Coliphages/genetics Escherichia coli/genetics,growth & development,metabolism Gene Expression Regulation, Bacterial Genes, Regulator Genotype Molecular Sequence Data Mutation Nitrates/metabolism Plasmids
Chemicals
Nitrates
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Egan S M
Section of Microbiology, Cornell University, Ithaca, New York 14853-8101.
Stewart V
References (33)
33 references, click to expand
  1. Nitrate reductase complex of Escherichia coli K-12: participation of specific formate dehydrogenase and cytochrome b1 components in nitrate reduction.
    J Bacteriol. 1969 Sep;99(3):720-9 PMID: 4905536
  2. Protein phosphorylation and regulation of adaptive responses in bacteria.
    Microbiol Rev. 1989 Dec;53(4):450-90 PMID: 2556636
  3. Mutants of Escherichia coli K12 unable to use fumarate as an anaerobic electron acceptor.
    J Gen Microbiol. 1976 Dec;97(2):145-60 PMID: 796407
  4. Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate.
    Anal Biochem. 1979 Sep 15;98(1):132-5 PMID: 543547
  5. Operon fusions in the nitrate reductase operon and study of the control gene nir R in Escherichia coli.
    Mol Gen Genet. 1981;182(3):477-9 PMID: 6457962
  6. Requirement of Fnr and NarL functions for nitrate reductase expression in Escherichia coli K-12.
    J Bacteriol. 1982 Sep;151(3):1320-5 PMID: 7050087
  7. In vitro insertional mutagenesis with a selectable DNA fragment.
    Gene. 1984 Sep;29(3):303-13 PMID: 6237955
  8. Site-directed insertion and deletion mutagenesis with cloned fragments in Escherichia coli.
    J Bacteriol. 1985 Mar;161(3):1219-21 PMID: 2982787
  9. A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.
    Proc Natl Acad Sci U S A. 1985 Feb;82(4):1074-8 PMID: 3156376
  10. Evidence for transcription antitermination control of tryptophanase operon expression in Escherichia coli K-12.
    J Bacteriol. 1985 Nov;164(2):731-40 PMID: 3902796
  11. Transcription of the Escherichia coli fumarate reductase genes (frdABCD) and their coordinate regulation by oxygen, nitrate, and fumarate.
    J Bacteriol. 1985 Dec;164(3):1100-9 PMID: 2999070
  12. Uses of lac fusions for the study of biological problems.
    Microbiol Rev. 1985 Dec;49(4):398-418 PMID: 3005818
  13. pHG165: a pBR322 copy number derivative of pUC8 for cloning and expression.
    Plasmid. 1986 May;15(3):172-81 PMID: 3012611
  14. Role of leader peptide synthesis in tryptophanase operon expression in Escherichia coli K-12.
    J Bacteriol. 1986 Jul;167(1):383-6 PMID: 3522554
  15. Structural analysis of the Escherichia coli K-12 hisT operon by using a kanamycin resistance cassette.
    J Bacteriol. 1987 Mar;169(3):1061-70 PMID: 3029016
  16. The narL gene product activates the nitrate reductase operon and represses the fumarate reductase and trimethylamine N-oxide reductase operons in Escherichia coli.
    Proc Natl Acad Sci U S A. 1987 Jun;84(11):3901-5 PMID: 3035558
  17. Improved single and multicopy lac-based cloning vectors for protein and operon fusions.
    Gene. 1987;53(1):85-96 PMID: 3596251
  18. Interposon mutagenesis of soil and water bacteria: a family of DNA fragments designed for in vitro insertional mutagenesis of gram-negative bacteria.
    Gene. 1987;52(2-3):147-54 PMID: 3038679
  19. Promoter region of the nar operon of Escherichia coli: nucleotide sequence and transcription initiation signals.
    J Bacteriol. 1987 Oct;169(10):4614-20 PMID: 3308846
  20. Molecular cloning of the wild-type phoM operon in Escherichia coli K-12.
    J Bacteriol. 1988 Jan;170(1):279-88 PMID: 3275616
  21. Identification and expression of genes narL and narX of the nar (nitrate reductase) locus in Escherichia coli K-12.
    J Bacteriol. 1988 Apr;170(4):1589-97 PMID: 2832370
  22. Polarity effects in the hisG gene of salmonella require a site within the coding sequence.
    Genetics. 1988 Feb;118(2):193-202 PMID: 3282985
  23. A simple and rapid method for the selection of oligodeoxynucleotide-directed mutants.
    Gene. 1988 May 15;65(1):129-33 PMID: 2840354
  24. Nitrate respiration in relation to facultative metabolism in enterobacteria.
    Microbiol Rev. 1988 Jun;52(2):190-232 PMID: 3045516
  25. Location of sequences in the nar promoter of Escherichia coli required for regulation by Fnr and NarL.
    J Biol Chem. 1988 Sep 25;263(27):13700-5 PMID: 3138237
  26. Influence of nar (nitrate reductase) genes on nitrate inhibition of formate-hydrogen lyase and fumarate reductase gene expression in Escherichia coli K-12.
    J Bacteriol. 1988 Oct;170(10):4437-44 PMID: 3049531
  27. Anaerobic regulation of pyruvate formate-lyase from Escherichia coli K-12.
    J Bacteriol. 1988 Nov;170(11):5330-6 PMID: 3053657
  28. Nucleotide sequence of the narL gene that is involved in global regulation of nitrate controlled respiratory genes of Escherichia coli.
    Nucleic Acids Res. 1989 Mar 11;17(5):1965-75 PMID: 2648330
  29. Structure of genes narL and narX of the nar (nitrate reductase) locus in Escherichia coli K-12.
    J Bacteriol. 1989 Apr;171(4):2229-34 PMID: 2649492
  30. The narX and narL genes encoding the nitrate-sensing regulators of Escherichia coli are homologous to a family of prokaryotic two-component regulatory genes.
    Nucleic Acids Res. 1989 Apr 25;17(8):2947-57 PMID: 2657652
  31. Identification of a second gene involved in global regulation of fumarate reductase and other nitrate-controlled genes for anaerobic respiration in Escherichia coli.
    J Bacteriol. 1989 Jul;171(7):3810-6 PMID: 2544557
  32. Oxygen, nitrate, and molybdenum regulation of dmsABC gene expression in Escherichia coli.
    J Bacteriol. 1989 Jul;171(7):3817-23 PMID: 2544558
  33. The role of a novel cytochrome b-containing nitrate reductase and quinone in the in vitro reconstruction of formate-nitrate reductase activity of E. coli.
    Biochem Biophys Res Commun. 1974 Dec 23;61(4):1234-41 PMID: 4616697
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.9.5020-5029.1990
Published
1990-09-00
Pages
5020-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC213158
Subset
IM
Grants
NIGMS NIH HHS · GM36877 · United States
External Links
PubMed source DOI: 10.1128/jb.172.9.5020-5029.1990 PMC Full Text View journal details
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