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PMID: 21333526 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Review

Near-atomic resolution reconstructions of icosahedral viruses from electron cryo-microscopy.

Current opinion in structural biology ·Vol. 21 ·No. 2 ·2011-04-00 ·Pages 265-73

Grigorieff N, Harrison SC

Abstract

Nine different near-atomic resolution structures of icosahedral viruses, determined by electron cryo-microscopy and published between early 2008 and late 2010, fulfil predictions made 15 years ago that single-particle cryo-EM techniques could visualize molecular detail at 3-4Å resolution. This review summarizes technical developments, both in instrumentation and in computation, that have led to the new structures, which advance our understanding of virus assembly and cell entry.

MeSH Terms
Computer Simulation Cryoelectron Microscopy Image Processing, Computer-Assisted Models, Molecular Virus Assembly Viruses/chemistry
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Grigorieff Nikolaus
Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA 02154, USA.
Harrison Stephen C
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Article Info
Journal
Current opinion in structural biology
Abbr.
Curr Opin Struct Biol
ISSN
1879-033X
DOI
10.1016/j.sbi.2011.01.008
Published
2011-04-00
Epub
2011-00-18
Pages
265-73
Language
English
Region
England
NLM ID
9107784
PMCID
PMC3088881
Subset
IM
Grants
NIGMS NIH HHS · P01 GM062580 · United States
NIGMS NIH HHS · R01 GM-62580 · United States
External Links
PubMed source DOI: 10.1016/j.sbi.2011.01.008 PMC Full Text View journal details
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