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PMID: 16216585 Published · ppublish English Comparative Study Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Features of reovirus outer capsid protein mu1 revealed by electron cryomicroscopy and image reconstruction of the virion at 7.0 Angstrom resolution.

Structure (London, England : 1993) ·Vol. 13 ·No. 10 ·2005-10-00 ·Pages 1545-57

Zhang X, Ji Y, Zhang L, Harrison SC, Marinescu DC, Nibert ML, Baker TS

Abstract

Reovirus is a useful model for addressing the molecular basis of membrane penetration by one of the larger nonenveloped animal viruses. We now report the structure of the reovirus virion at approximately 7.0 A resolution as obtained by electron cryomicroscopy and three-dimensional image reconstruction. Several features of the myristoylated outer capsid protein mu1, not seen in a previous X-ray crystal structure of the mu1-sigma3 heterohexamer, are evident in the virion. These features appear to be important for stabilizing the outer capsid, regulating the conformational changes in mu1 that accompany perforation of target membranes, and contributing directly to membrane penetration during cell entry.

MeSH Terms
Algorithms Amino Acid Sequence Animals Capsid Proteins/chemistry,isolation & purification,ultrastructure Cryoelectron Microscopy Crystallography, X-Ray Fourier Analysis L Cells Mice Models, Chemical Models, Molecular Molecular Sequence Data Myristic Acid/metabolism Orthoreovirus/chemistry,genetics,ultrastructure Protein Conformation Protein Structure, Secondary Sequence Homology, Amino Acid Virion/growth & development,ultrastructure
Chemicals
Capsid Proteins Myristic Acid
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Zhang Xing
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA.
Ji Yongchang
Zhang Lan
Harrison Stephen C
Marinescu Dan C
Nibert Max L
Baker Timothy S
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Article Info
Journal
Structure (London, England : 1993)
Abbr.
Structure
ISSN
0969-2126
DOI
10.1016/j.str.2005.07.012
Published
2005-10-00
Pages
1545-57
Language
English
Region
United States
NLM ID
101087697
PMCID
PMC4126556
Subset
IM
Grants
NIGMS NIH HHS · R37 GM033050 · United States
NIGMS NIH HHS · P01 GM062580 · United States
NIGMS NIH HHS · R01 GM033050 · United States
NCI NIH HHS · R37 CA013202 · United States
NIAID NIH HHS · P01 AI045976 · United States
NIAID NIH HHS · R01 AI046440 · United States
NIAID NIH HHS · R01 AI47904 · United States
NIGMS NIH HHS · R01 GM33050 · United States
NIAID NIH HHS · R01 AI46440 · United States
NCI NIH HHS · R01 CA013202 · United States
NIAID NIH HHS · R01 AI047904 · United States
NIGMS NIH HHS · R01 GM62580 · United States
NCI NIH HHS · R01 CA13202 · United States
Databases
PDB
2CSE
External Links
PubMed source DOI: 10.1016/j.str.2005.07.012 PMC Full Text View journal details
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