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PMID: 21317392 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Highly activated cytotoxic CD8 T cells express protective IL-10 at the peak of coronavirus-induced encephalitis.

Journal of immunology (Baltimore, Md. : 1950) ·Vol. 186 ·No. 6 ·2011-03-15 ·Pages 3642-52

Trandem K, Zhao J, Fleming E, Perlman S

Abstract

Acute viral encephalitis requires rapid pathogen elimination without significant bystander tissue damage. In this article, we show that IL-10, a potent anti-inflammatory cytokine, is produced transiently at the peak of infection by CD8 T cells in the brains of coronavirus-infected mice. IL-10(+)CD8 and IL-10(-)CD8 T cells interconvert during acute disease, possibly based on recent Ag exposure. Strikingly, IL-10(+)CD8 T cells were more highly activated and cytolytic than IL-10(-)CD8 T cells, expressing greater levels of proinflammatory cytokines and chemokines, as well as cytotoxic proteins. Even though these cells are highly proinflammatory, IL-10 expressed by these cells was functional. Furthermore, IL-10 produced by CD8 T cells diminished disease severity in mice with coronavirus-induced acute encephalitis, suggesting a self-regulatory mechanism that minimizes immunopathological changes.

MeSH Terms
Adoptive Transfer Animals Cell Line, Tumor Cells, Cultured Coronaviridae Infections/immunology,metabolism,prevention & control Cytotoxicity, Immunologic Encephalomyelitis, Acute Disseminated/immunology,pathology,prevention & control Inflammation Mediators/metabolism Interleukin-10/biosynthesis,deficiency,physiology Lymphocyte Activation/immunology Mice Mice, Inbred C57BL Mice, Knockout Mice, Transgenic Murine hepatitis virus/immunology T-Lymphocytes, Cytotoxic/immunology,metabolism,transplantation
Chemicals
IL10 protein, mouse Inflammation Mediators Interleukin-10
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Trandem Kathryn
Interdisciplinary Program in Immunology, University of Iowa, Iowa City, IA 52242, USA.
Zhao Jingxian
Fleming Erica
Perlman Stanley
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Article Info
Journal
Journal of immunology (Baltimore, Md. : 1950)
Abbr.
J Immunol
ISSN
1550-6606
DOI
10.4049/jimmunol.1003292
Published
2011-03-15
Epub
2011-00-11
Pages
3642-52
Language
English
Region
United States
NLM ID
2985117R
PMCID
PMC3063297
Subset
IM
Grants
NINDS NIH HHS · R01 NS036592 · United States
NINDS NIH HHS · R01 NS036592-13 · United States
NINDS NIH HHS · NS36592 · United States
NIAID NIH HHS · T32 AI007511 · United States
NIGMS NIH HHS · T32 GM007337 · United States
NIAID NIH HHS · T32 AI007511-14 · United States
Databases
GEO
GSE25846
External Links
PubMed source DOI: 10.4049/jimmunol.1003292 PMC Full Text View journal details
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