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PMID: 21283722 Published · epublish English Journal Article Research Support, N.I.H., Extramural

The CCCTC-binding factor (CTCF) of Drosophila contributes to the regulation of the ribosomal DNA and nucleolar stability.

PloS one ·Vol. 6 ·No. 1 ·2011-01-20 ·Pages e16401

Guerrero PA, Maggert KA

Abstract

In the repeat array of ribosomal DNA (rDNA), only about half of the genes are actively transcribed while the others are silenced. In arthropods, transposable elements interrupt a subset of genes, often inactivating transcription of those genes. Little is known about the establishment or separation of juxtaposed active and inactive chromatin domains, or preferential inactivation of transposable element interrupted genes, despite identity in promoter sequences. CTCF is a sequence-specific DNA binding protein which is thought to act as a transcriptional repressor, block enhancer-promoter communication, and delimit juxtaposed domains of active and inactive chromatin; one or more of these activities might contribute to the regulation of this repeated gene cluster. In support of this hypothesis, we show that the Drosophila nucleolus contains CTCF, which is bound to transposable element sequences within the rDNA. Reduction in CTCF gene activity results in nucleolar fragmentation and reduced rDNA silencing, as does disruption of poly-ADP-ribosylation thought to be necessary for CTCF nucleolar localization. Our data establish a role for CTCF as a component necessary for proper control of transposable element-laden rDNA transcription and nucleolar stability.

MeSH Terms
Animals CCCTC-Binding Factor Cell Nucleolus/chemistry DNA Transposable Elements DNA, Ribosomal/genetics Drosophila melanogaster/genetics Gene Silencing Protein Binding Repressor Proteins/analysis,physiology
Chemicals
CCCTC-Binding Factor DNA Transposable Elements DNA, Ribosomal Repressor Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Guerrero Paola A
Department of Biochemistry/Biophysics, Texas A&M University, College Station, Texas, United States of America.
Maggert Keith A
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Article Info
Journal
PloS one
Abbr.
PLoS One
ISSN
1932-6203
DOI
10.1371/journal.pone.0016401
Published
2011-01-20
Epub
2011-00-20
Pages
e16401
Language
English
Region
United States
NLM ID
101285081
PMCID
PMC3024428
Subset
IM
Grants
NIGMS NIH HHS · R01 GM076092 · United States
External Links
PubMed source DOI: 10.1371/journal.pone.0016401 PMC Full Text View journal details
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