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PMID: 2117569 Published · ppublish English Journal Article

Up-regulation of human neutrophil receptors for Neisseria gonorrhoeae expressing PII outer membrane proteins.

Infection and immunity ·Vol. 58 ·No. 9 ·1990-09-00 ·Pages 2777-84

Farrell CF, Rest RF

Abstract

In the absence of serum, nonpiliated gonococci expressing PII outer membrane proteins (PIIs) adhere to human neutrophils whereas non-PII-expressing (PII-) gonococci do not. After an observation that neutrophils in monolayers bound more gonococci than neutrophils in suspension, we treated neutrophil suspensions with known stimulants of degranulation and measured subsequent gonococcal adherence to suspended neutrophils. The chemotactic peptide N-formyl-methionyl-leucyl-phenylalanine (fmlp), the potent secretagogue phorbol myristate acetate, and the calcium ionophore A23187 all caused increased adherence of PII+ gonococci, but not PII- gonococci, to neutrophils in a dose-responsive manner. Increased adherence of gonococci to neutrophils was paralleled by increased degranulation of neutrophil myeloperoxidase, lysozyme, and lactoferrin. Inhibition of fmlp-induced neutrophil degranulation by pertussis toxin, the calmodulin inhibitors trifluoperazine and N-5-chloronaphthalene sulfonamide, or the intracellular calcium-binding agent trimethoxybenzoic acid also inhibited fmlp-induced gonococcal adherence to neutrophils. Neither undifferentiated nor myelocytically differentiated HL-60 cells, which possess primary but defective or nonexistent secondary granules, bound PII+ or PII- gonococci. Gonococci did not adhere to human monocytes, monocyte-derived macrophages, lymphocytes, platelets, or erythrocytes, indicating that several receptors, such as the complement receptors CR1, CR3 (CD11b/CD18), and CR4 (CD11c/CD18) or the adherence complex LFA-1 (CD11a/CD18), were probably not involved in gonococcal adherence to human neutrophils.

MeSH Terms
Antibodies, Monoclonal/immunology Antigens, Bacterial/biosynthesis,immunology Bacterial Adhesion/drug effects,immunology Calcium Channel Blockers/pharmacology Calmodulin/antagonists & inhibitors Gallic Acid/analogs & derivatives,pharmacology Humans Neisseria gonorrhoeae/immunology,metabolism Neutrophils/drug effects,metabolism Pertussis Toxin Sulfonamides/pharmacology Tetradecanoylphorbol Acetate/pharmacology Trifluoperazine/pharmacology Up-Regulation Virulence Factors, Bordetella/pharmacology
Chemicals
Antibodies, Monoclonal Antigens, Bacterial Calcium Channel Blockers Calmodulin Sulfonamides Virulence Factors, Bordetella opacity proteins Trifluoperazine 8-(N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate Gallic Acid W 7 Pertussis Toxin Tetradecanoylphorbol Acetate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Farrell C F
Department of Microbiology and Immunology, Hahnemann University School of Medicine, Philadelphia, Pennsylvania 19102-1192.
Rest R F
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.58.9.2777-2784.1990
Published
1990-09-00
Pages
2777-84
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC313567
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.58.9.2777-2784.1990 PMC Full Text View journal details
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