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PMID: 3930402 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Stimulation of human leukocytes by protein II+ gonococci is mediated by lectin-like gonococcal components.

Infection and immunity ·Vol. 50 ·No. 1 ·1985-10-00 ·Pages 116-22

Rest RF, Lee N, Bowden C

Abstract

We investigated the ability of carbohydrates, glycosidases, and concanavalin A to inhibit the stimulation of the human leukocyte oxidative burst by gonococci in the absence of serum. The gonococci used in this study contained protein II (P.II) outer membrane proteins, and neutrophil oxidative burst was measured by luminol-enhanced chemiluminescence (CL). The following carbohydrates inhibited CL induced by nonpiliated P.II+ gonococci: beta-D-glucosamine greater than N-acetylneuraminic acid (sialic acid) greater than mannose greater than alpha-methylmannoside greater than N-acetyl-beta-D-glucosamine greater than or equal to glucose greater than or equal to lactose. Fucose, galactose, or beta-D-galactosamine (all 100 mM) did not inhibit or slightly increased CL, indicating a specificity for the observed effects. Mannose and alpha-methylmannoside also inhibited induction of monocyte CL by P.II+ gonococci. Incubation of neutrophils with concanavalin A inhibited subsequent gonococcus-induced CL but not phorbol myristate acetate-induced CL. Treatment of neutrophils with alpha-mannosidase reduced subsequent gonococcus-induced CL greater than 99%, whereas such treatment of gonococci had no effect on their ability to induce neutrophil CL. Incubation of a P.IIb-containing variant of Neisseria gonorrhoeae FA1090 with anti-P.IIb monoclonal antibody inhibited subsequent stimulation of neutrophil CL in a dose-responsive manner, indicating a specific role for P.IIb in the stimulatory process. The data suggest that one or more lectin-like components on the surface of P.II+ gonococci mediate their ability to stimulate the oxidative burst of human phagocytes.

MeSH Terms
Antibodies, Monoclonal Bacterial Outer Membrane Proteins/immunology Carbohydrates/pharmacology Concanavalin A/immunology Glycoside Hydrolases Humans Immunity, Cellular Luminescent Measurements Neisseria gonorrhoeae/immunology Neutrophils/immunology Oxygen Consumption
Chemicals
Antibodies, Monoclonal Bacterial Outer Membrane Proteins Carbohydrates Concanavalin A Glycoside Hydrolases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rest R F
Lee N
Bowden C
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52 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.50.1.116-122.1985
Published
1985-10-00
Pages
116-22
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC262144
Subset
IM
Grants
NIAID NIH HHS · AI00594 · United States
NIAID NIH HHS · AI14934 · United States
NIAID NIH HHS · AI20894 · United States
External Links
PubMed source DOI: 10.1128/iai.50.1.116-122.1985 PMC Full Text View journal details
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