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PMID: 21131278 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Translation on demand by a simple RNA-based thermosensor.

Nucleic acids research ·Vol. 39 ·No. 7 ·2011-04-00 ·Pages 2855-68

Kortmann J, Sczodrok S, Rinnenthal J, Schwalbe H, Narberhaus F

Abstract

Structured RNA regions are important gene control elements in prokaryotes and eukaryotes. Here, we show that the mRNA of a cyanobacterial heat shock gene contains a built-in thermosensor critical for photosynthetic activity under stress conditions. The exceptionally short 5'-untranslated region is comprised of a single hairpin with an internal asymmetric loop. It inhibits translation of the Synechocystis hsp17 transcript at normal growth conditions, permits translation initiation under stress conditions and shuts down Hsp17 production in the recovery phase. Point mutations that stabilized or destabilized the RNA structure deregulated reporter gene expression in vivo and ribosome binding in vitro. Introduction of such point mutations into the Synechocystis genome produced severe phenotypic defects. Reversible formation of the open and closed structure was beneficial for viability, integrity of the photosystem and oxygen evolution. Continuous production of Hsp17 was detrimental when the stress declined indicating that shutting-off heat shock protein production is an important, previously unrecognized function of RNA thermometers. We discovered a simple biosensor that strictly adjusts the cellular level of a molecular chaperone to the physiological need.

MeSH Terms
5' Untranslated Regions Bacterial Proteins/genetics Heat-Shock Proteins/genetics Photosynthesis/genetics Point Mutation Protein Biosynthesis Ribosomes/metabolism Synechocystis/genetics,growth & development,metabolism Temperature
Chemicals
5' Untranslated Regions Bacterial Proteins Heat-Shock Proteins HspA protein, bacteria
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kortmann Jens
Lehrstuhl für Biologie der Mikroorganismen, Ruhr-Universität Bochum, 44780 Bochum, Germany.
Sczodrok Simon
Rinnenthal Jörg
Schwalbe Harald
Narberhaus Franz
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
Published
2011-04-00
Epub
2010-00-03
Pages
2855-68
Language
English
Region
England
NLM ID
0411011
PMCID
PMC3074152
Subset
IM
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