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LiteratureArticle Details
PMID: 20952648
Published · ppublish
English
Journal Article
Research Support, Non-U.S. Gov't
Both leaf properties and microbe-microbe interactions influence within-species variation in bacterial population diversity and structure in the lettuce (Lactuca Species) phyllosphere.
Abstract
Morphological and chemical differences between plant genera influence phyllosphere microbial populations, but the factors driving within-species variation in phyllosphere populations are poorly understood. Twenty-six lettuce accessions were used to investigate factors controlling within-species variation in phyllosphere bacterial populations. Morphological and physiochemical characteristics of the plants were compared, and bacterial community structure and diversity were investigated using terminal restriction fragment length polymorphism (T-RFLP) profiling and 16S rRNA gene clone libraries. Plant morphology and levels of soluble carbohydrates, calcium, and phenolic compounds (which have long been associated with plant responses to biotic stress) were found to significantly influence bacterial community structure. Clone libraries from three representative accessions were found to be significantly different in terms of both sequence differences and the bacterial genera represented. All three libraries were dominated by Pseudomonas species and the Enterobacteriaceae family. Significant differences in the relative proportions of genera in the Enterobacteriaceae were detected between lettuce accessions. Two such genera (Erwinia and Enterobacter) showed significant variation between the accessions and revealed microbe-microbe interactions. We conclude that both leaf surface properties and microbial interactions are important in determining the structure and diversity of the phyllosphere bacterial community.
MeSH Terms
Biodiversity
Calcium/analysis
Carbohydrates/analysis
Cluster Analysis
DNA Fingerprinting
DNA, Bacterial/chemistry,genetics
DNA, Ribosomal/chemistry,genetics
Lettuce/microbiology
Microbial Interactions
Molecular Sequence Data
Phenols/analysis
Phylogeny
Plant Leaves/chemistry,microbiology
Polymorphism, Restriction Fragment Length
RNA, Ribosomal, 16S/genetics
Sequence Analysis, DNA
Chemicals
Carbohydrates
DNA, Bacterial
DNA, Ribosomal
Phenols
RNA, Ribosomal, 16S
Calcium
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
1098-5336
Published
2010-12-00
Epub
2010-00-15
Pages
8117-25
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC3008232
Subset
IM
Databases
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