Microbial phyllosphere populations are more complex than previously realized.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 98 ·No. 7 ·2001-03-27 ·Pages 3889-94

Yang CH, Crowley DE, Borneman J, Keen NT

Abstract

Phyllosphere microbial communities were evaluated on leaves of field-grown plant species by culture-dependent and -independent methods. Denaturing gradient gel electrophoresis (DGGE) with 16S rDNA primers generally indicated that microbial community structures were similar on different individuals of the same plant species, but unique on different plant species. Phyllosphere bacteria were identified from Citrus sinesis (cv. Valencia) by using DGGE analysis followed by cloning and sequencing of the dominant rDNA bands. Of the 17 unique sequences obtained, database queries showed only four strains that had been described previously as phyllosphere bacteria. Five of the 17 sequences had 16S similarities lower than 90% to database entries, suggesting that they represent previously undescribed species. In addition, three fungal species were also identified. Very different 16S rDNA DGGE banding profiles were obtained when replicate cv. Valencia leaf samples were cultured in BIOLOG EcoPlates for 4.5 days. All of these rDNA sequences had 97--100% similarity to those of known phyllosphere bacteria, but only two of them matched those identified by the culture independent DGGE analysis. Like other studied ecosystems, microbial phyllosphere communities therefore are more complex than previously thought, based on conventional culture-based methods.

MeSH Terms

Bacteria/classification,genetics Citrus/microbiology Culture Media DNA, Bacterial/analysis Molecular Sequence Data Plant Leaves/microbiology Polymerase Chain Reaction RNA, Ribosomal, 16S/analysis,classification

Chemicals

Culture Media DNA, Bacterial RNA, Ribosomal, 16S

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Yang C H

Department of Plant Pathology, University of California, Riverside, CA 92521, USA.

Crowley D E

Borneman J

Keen N T

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18 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.051633898

Published

2001-03-27

Pages

3889-94

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC31148

Subset

Databases

GENBANK

AF231471, AF231472, AF231473, AF231474, AF231475, AF231476, AF231477, AF231478, AF231479, AF231480, AF231481, AF231482, AF231483, AF231484, AF231485, AF231486, AF231487, AF231488, AF231489, AF231490, AF231491, AF231492, AF231493, AF231494, AF231495, AF231496, AF231497

External Links

PubMed source DOI: 10.1073/pnas.051633898 PMC Full Text View journal details

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