Abstract
Chaperone-mediated autophagy (CMA) is a selective mechanism for the degradation of cytosolic proteins in lysosomes that contributes to cellular quality control and becomes an additional source of amino acids when nutrients are scarce. A chaperone complex delivers CMA substrates to a receptor protein at the lysosomal membrane that assembles into multimeric translocation complexes. However, the mechanisms regulating this process remain, for the most part, unknown. In this work, we have identified two regulatory proteins, GFAP and EF1alpha, that mediate a previously unknown inhibitory effect of GTP on CMA. GFAP stabilizes the multimeric translocation complex against chaperone-mediated disassembly, whereas GTP-mediated release of EF1alpha from the lysosomal membrane promotes self-association of GFAP, disassembly of the CMA translocation complex, and the consequent decrease in CMA. The dynamic interactions of these two proteins at the lysosomal membrane unveil now a role for GTP as a negative regulator of CMA.
MeSH Terms
Animals
Autophagy
Fibroblasts/metabolism,pathology
Glial Fibrillary Acidic Protein
Guanosine Triphosphate/metabolism
Hepatocytes/metabolism,pathology
Lysosomal-Associated Membrane Protein 2/genetics,metabolism
Lysosomes/metabolism
Male
Mice
Molecular Chaperones/metabolism
Multiprotein Complexes
NIH 3T3 Cells
Nerve Tissue Proteins/metabolism
Peptide Elongation Factor 1/metabolism
Protein Transport
RNA Interference
Rats
Rats, Sprague-Dawley
Time Factors
Transfection
Chemicals
Glial Fibrillary Acidic Protein
Lysosomal-Associated Membrane Protein 2
Molecular Chaperones
Multiprotein Complexes
Nerve Tissue Proteins
Peptide Elongation Factor 1
glial fibrillary astrocytic protein, mouse
Guanosine Triphosphate
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Bandyopadhyay Urmi
Department of Development and Molecular Biology, Institute for Aging Studies, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
Sridhar Sunandini
Kaushik Susmita
Kiffin Roberta
Cuervo Ana Maria
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