Kick-sTARting HIV-1 transcription elongation by 7SK snRNP deporTATion.

Nature structural & molecular biology ·Vol. 17 ·No. 8 ·2010-08-00 ·Pages 928-30

Barboric M, Lenasi T

Abstract

The HIV-1 Tat protein promotes viral transcription elongation by recruiting P-TEFb to RNA element TAR on the viral mRNA. Recent work from D'Orso and Frankel uncovers unexpected aspects of this process.

MeSH Terms

Gene Expression Regulation, Viral HIV Long Terminal Repeat/genetics HIV-1/genetics Models, Genetic Positive Transcriptional Elongation Factor B/metabolism RNA Polymerase II/metabolism Ribonucleoproteins, Small Nuclear/metabolism Transcription, Genetic Transcriptional Activation/genetics tat Gene Products, Human Immunodeficiency Virus/genetics,metabolism

Chemicals

Ribonucleoproteins, Small Nuclear tat Gene Products, Human Immunodeficiency Virus Positive Transcriptional Elongation Factor B RNA Polymerase II

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Barboric Matjaz

Lenasi Tina

References (38)

38 references, click to expand

A La-related protein modulates 7SK snRNP integrity to suppress P-TEFb-dependent transcriptional elongation and tumorigenesis.
Mol Cell. 2008 Mar 14;29(5):588-99 PMID: 18249148
LARP7 is a stable component of the 7SK snRNP while P-TEFb, HEXIM1 and hnRNP A1 are reversibly associated.
Nucleic Acids Res. 2008 Apr;36(7):2219-29 PMID: 18281698
Tat competes with HEXIM1 to increase the active pool of P-TEFb for HIV-1 transcription.
Nucleic Acids Res. 2007;35(6):2003-12 PMID: 17341462
T-loop phosphorylated Cdk9 localizes to nuclear speckle domains which may serve as sites of active P-TEFb function and exchange between the Brd4 and 7SK/HEXIM1 regulatory complexes.
J Cell Physiol. 2010 Jul;224(1):84-93 PMID: 20201073
Progression through the RNA polymerase II CTD cycle.
Mol Cell. 2009 Nov 25;36(4):541-6 PMID: 19941815
Crystal structure of HIV-1 Tat complexed with human P-TEFb.
Nature. 2010 Jun 10;465(7299):747-51 PMID: 20535204
HIV-1 Tat protein interacts with mammalian capping enzyme and stimulates capping of TAR RNA.
J Biol Chem. 2001 Apr 20;276(16):12959-66 PMID: 11278368
The transcription-dependent dissociation of P-TEFb-HEXIM1-7SK RNA relies upon formation of hnRNP-7SK RNA complexes.
Mol Cell Biol. 2007 Oct;27(20):6996-7006 PMID: 17709395
Identification of a cyclin T-binding domain in Hexim1 and biochemical analysis of its binding competition with HIV-1 Tat.
J Biol Chem. 2005 Jul 1;280(26):24968-77 PMID: 15855166
Structural insights into the cyclin T1-Tat-TAR RNA transcription activation complex from EIAV.
Nat Struct Mol Biol. 2008 Dec;15(12):1287-92 PMID: 19029897
Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme.
Mol Cell. 2007 Jul 20;27(2):262-74 PMID: 17643375
Conserved P-TEFb-interacting domain of BRD4 inhibits HIV transcription.
Proc Natl Acad Sci U S A. 2007 Aug 21;104(34):13690-5 PMID: 17690245
Domains in the SPT5 protein that modulate its transcriptional regulatory properties.
Mol Cell Biol. 2000 May;20(9):2970-83 PMID: 10757782
HIV-1 Tat assembles a multifunctional transcription elongation complex and stably associates with the 7SK snRNP.
Mol Cell. 2010 May 14;38(3):439-51 PMID: 20471949
The human immunodeficiency virus transactivator Tat interacts with the RNA polymerase II holoenzyme.
Mol Cell Biol. 1997 Apr;17(4):1817-23 PMID: 9121429
Poised RNA polymerase II gives pause for thought.
Cell. 2008 May 16;133(4):581-4 PMID: 18485867
7SK RNA, a non-coding RNA regulating P-TEFb, a general transcription factor.
RNA Biol. 2009 Apr-Jun;6(2):122-8 PMID: 19246988
Recruitment of P-TEFb for stimulation of transcriptional elongation by the bromodomain protein Brd4.
Mol Cell. 2005 Aug 19;19(4):535-45 PMID: 16109377
Dynamic remodelling of human 7SK snRNP controls the nuclear level of active P-TEFb.
EMBO J. 2007 Aug 8;26(15):3570-80 PMID: 17611602
Sp1-dependent activation of a synthetic promoter by human immunodeficiency virus type 1 Tat protein.
Proc Natl Acad Sci U S A. 1991 Oct 1;88(19):8510-4 PMID: 1924310
NELF, a multisubunit complex containing RD, cooperates with DSIF to repress RNA polymerase II elongation.
Cell. 1999 Apr 2;97(1):41-51 PMID: 10199401
NF-kappaB binds P-TEFb to stimulate transcriptional elongation by RNA polymerase II.
Mol Cell. 2001 Aug;8(2):327-37 PMID: 11545735
Tat modifies the activity of CDK9 to phosphorylate serine 5 of the RNA polymerase II carboxyl-terminal domain during human immunodeficiency virus type 1 transcription.
Mol Cell Biol. 2000 Jul;20(14):5077-86 PMID: 10866664
Sustained induction of NF-kappa B is required for efficient expression of latent human immunodeficiency virus type 1.
J Virol. 2007 Jun;81(11):6043-56 PMID: 17376917
A novel CDK9-associated C-type cyclin interacts directly with HIV-1 Tat and mediates its high-affinity, loop-specific binding to TAR RNA.
Cell. 1998 Feb 20;92(4):451-62 PMID: 9491887
DSIF, the Paf1 complex, and Tat-SF1 have nonredundant, cooperative roles in RNA polymerase II elongation.
Genes Dev. 2009 Dec 1;23(23):2765-77 PMID: 19952111
HIV-1 Tat and host AFF4 recruit two transcription elongation factors into a bifunctional complex for coordinated activation of HIV-1 transcription.
Mol Cell. 2010 May 14;38(3):428-38 PMID: 20471948
Control of inducible gene expression by signal-dependent transcriptional elongation.
Cell. 2009 Jul 10;138(1):129-45 PMID: 19596240
Structure and mechanism of the RNA polymerase II transcription machinery.
Nat Struct Mol Biol. 2004 May;11(5):394-403 PMID: 15114340
Controlling the elongation phase of transcription with P-TEFb.
Mol Cell. 2006 Aug 4;23(3):297-305 PMID: 16885020
RNA-mediated displacement of an inhibitory snRNP complex activates transcription elongation.
Nat Struct Mol Biol. 2010 Jul;17(7):815-21 PMID: 20562857
P-TEFb stimulates transcription elongation and pre-mRNA splicing through multilateral mechanisms.
RNA Biol. 2010 Mar-Apr;7(2):145-50 PMID: 20305375
Dynamics of human immunodeficiency virus transcription: P-TEFb phosphorylates RD and dissociates negative effectors from the transactivation response element.
Mol Cell Biol. 2004 Jan;24(2):787-95 PMID: 14701750
7SK snRNP/P-TEFb couples transcription elongation with alternative splicing and is essential for vertebrate development.
Proc Natl Acad Sci U S A. 2009 May 12;106(19):7798-803 PMID: 19416841
Manipulation of P-TEFb control machinery by HIV: recruitment of P-TEFb from the large form by Tat and binding of HEXIM1 to TAR.
Nucleic Acids Res. 2007;35(13):4347-58 PMID: 17576689
Tat-associated kinase (P-TEFb): a component of transcription preinitiation and elongation complexes.
J Biol Chem. 1999 Mar 12;274(11):7399-404 PMID: 10066804
Association of Tat with purified HIV-1 and HIV-2 transcription preinitiation complexes.
J Biol Chem. 1997 Mar 14;272(11):6951-8 PMID: 9054383
CDK8 is a positive regulator of transcriptional elongation within the serum response network.
Nat Struct Mol Biol. 2010 Feb;17(2):194-201 PMID: 20098423

Article Info

Journal

Nature structural & molecular biology

Abbr.

Nat Struct Mol Biol

ISSN

1545-9985

DOI

10.1038/nsmb0810-928

Published

2010-08-00

Pages

928-30

Language

English

Region

United States

NLM ID

101186374

Subset

Corrections

CommentOn

PMID: 20562857

External Links

PubMed source DOI: 10.1038/nsmb0810-928 View journal details

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Kick-sTARting HIV-1 transcription elongation by 7SK snRNP deporTATion.

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