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PMID: 20622844 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Review

Proteomics: a pragmatic perspective.

Nature biotechnology ·Vol. 28 ·No. 7 ·2010-07-00 ·Pages 695-709

Mallick P, Kuster B

Abstract

The evolution of mass spectrometry-based proteomic technologies has advanced our understanding of the complex and dynamic nature of proteomes while concurrently revealing that no 'one-size-fits-all' proteomic strategy can be used to address all biological questions. Whereas some techniques, such as those for analyzing protein complexes, have matured and are broadly applied with great success, others, such as global quantitative protein expression profiling for biomarker discovery, are still confined to a few expert laboratories. In this Perspective, we attempt to distill the wide array of conceivable proteomic approaches into a compact canon of techniques suited to asking and answering specific types of biological questions. By discussing the relationship between the complexity of a biological sample and the difficulty of implementing the appropriate analysis approach, we contrast areas of proteomics broadly usable today with those that require significant technical and conceptual development. We hope to provide nonexperts with a guide for calibrating expectations of what can realistically be learned from a proteomics experiment and for gauging the planning and execution effort. We further provide a detailed supplement explaining the most common techniques in proteomics.

MeSH Terms
Cells, Cultured Gene Expression Profiling Humans Organelles/metabolism Proteomics Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mallick Parag
University of Southern California Center for Applied Molecular Medicine, Departments of Medicine and Biomedical Engineering, Los Angeles, California, USA. mallick@usc.edu
Kuster Bernhard
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Article Info
Journal
Nature biotechnology
Abbr.
Nat Biotechnol
ISSN
1546-1696
DOI
10.1038/nbt.1658
Email
mallick@usc.edu
Published
2010-07-00
Epub
2010-00-09
Pages
695-709
Language
English
Region
United States
NLM ID
9604648
Subset
IM
Grants
NCI NIH HHS · 1U54CA143907 · United States
NCI NIH HHS · U54CA119367 · United States
External Links
PubMed source DOI: 10.1038/nbt.1658 View journal details
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