Home LiteratureArticle Details
PMID: 2032421 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Expression and role of integrins in adhesion of human colonic carcinoma cells to extracellular matrix components.

Clinical & experimental metastasis ·Vol. 9 ·No. 2 ·1991-00-00 ·Pages 163-78

Schreiner C, Bauer J, Margolis M, Juliano RL

Abstract

We have examined integrin expression and function in the human colon carcinoma cell line HT29, and in clonal sublines derived from the HT29 line. These cells express several different integrin subunits including beta 1, alpha 2, 3, 6 and alpha v, but do not express the classic alpha 5/beta 1 fibronectin receptor. Clonal variation in the pattern of integrin expression was quite limited. The profile of integrin expression correlates well with the adhesive behavior of HT29 cells. Thus the cells adhere well to vitronectin, laminin and type IV collagen, but not at all to fibronectin. Adhesion to collagen was completely blocked by an anti-beta 1 monoclonal antibody, indicating that beta 1 integrins mediate this process. Adhesion to laminin was strongly blocked by anti-beta 1 monoclonal or anti-beta 6 monoclonal, suggesting that the alpha 6/beta 1 complex functions in attachment to laminin; this was somewhat surprising since immunoprecipitation experiments indicate that most of the alpha 6 subunit seems to be associated with the beta 4 subunit. Despite their strong adherence to laminin, collagen and vitronectin, HT29 cells are not very motile and, in response to gradients of these proteins, do not migrate nearly as well as CHO cells tested under similar conditions. Since HT29 cells can undergo an enterocyte-like differentiation in glucose-free medium, we compared integrin expression in HT29 and its subclones during the process of differentiation. There was no correlation between the state of differentiation, as assessed by expression of brush-border hydrolases, and the level of expression of any of the integrin subunits measured. Thus the pattern of integrin expression in these colonic tumor cells seems to be a characteristic of the cell line, and is not readily modified by changes in cell growth or differentiation.

MeSH Terms
Carcinoma/chemistry,pathology Cell Adhesion Cell Differentiation Cell Movement Colonic Neoplasms/chemistry,pathology Extracellular Matrix/pathology Humans Integrins/analysis,physiology Tumor Cells, Cultured
Chemicals
Integrins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Schreiner C
Department of Pharmacology, School of Medicine, University of North Carolina, Chapel Hill 27599.
Bauer J
Margolis M
Juliano R L
References (58)
58 references, click to expand
  1. Influence of stereochemistry of the sequence Arg-Gly-Asp-Xaa on binding specificity in cell adhesion.
    J Biol Chem. 1987 Dec 25;262(36):17294-8 PMID: 3693352
  2. Elevated levels of the alpha 5 beta 1 fibronectin receptor suppress the transformed phenotype of Chinese hamster ovary cells.
    Cell. 1990 Mar 9;60(5):849-59 PMID: 2155708
  3. Colon and rectal carcinoma.
    Curr Probl Cancer. 1987 Sep-Oct;11(5):257-356 PMID: 3319428
  4. Changes in integrin receptors on oncogenically transformed cells.
    Cell. 1989 Jan 27;56(2):281-90 PMID: 2521461
  5. Recent hypotheses for the origin of colon cancer.
    Cancer Res. 1987 Aug 15;47(16):4237-42 PMID: 3300962
  6. Integrins: a family of cell surface receptors.
    Cell. 1987 Feb 27;48(4):549-54 PMID: 3028640
  7. Cell adhesion protein receptors as targets for transforming growth factor-beta action.
    Cell. 1987 Oct 23;51(2):189-97 PMID: 3499229
  8. Failure of expression of class I major histocompatibility antigens to alter tumor immunogenicity of a spontaneous murine carcinoma.
    J Natl Cancer Inst. 1989 May 10;81(10):759-67 PMID: 2654405
  9. METHOD FOR ASSAY OF INTESTINAL DISACCHARIDASES.
    Anal Biochem. 1964 Jan;7:18-25 PMID: 14106916
  10. Selective inhibition of fibronectin-mediated cell adhesion by monoclonal antibodies to a cell-surface glycoprotein.
    Science. 1985 Jun 21;228(4706):1448-51 PMID: 4012302
  11. Human melanoma cells express a novel integrin receptor for laminin.
    J Biol Chem. 1989 Sep 15;264(26):15642-9 PMID: 2527855
  12. Epithelial polarity, villin expression, and enterocytic differentiation of cultured human colon carcinoma cells: a survey of twenty cell lines.
    Cancer Res. 1988 Apr 1;48(7):1936-42 PMID: 3349466
  13. A fine-structure genetic and chemical study of the enzyme alkaline phosphatase of E. coli. I. Purification and characterization of alkaline phosphatase.
    Biochim Biophys Acta. 1960 Mar 11;38:470-83 PMID: 13826559
  14. cDNA and amino acid sequences of the cell adhesion protein receptor recognizing vitronectin reveal a transmembrane domain and homologies with other adhesion protein receptors.
    Proc Natl Acad Sci U S A. 1986 Nov;83(22):8614-8 PMID: 2430295
  15. Site-directed mutagenesis of the cell-binding domain of human fibronectin: separable, synergistic sites mediate adhesive function.
    Cell. 1988 May 20;53(4):649-57 PMID: 3286012
  16. Identification of multiple cell adhesion receptors for collagen and fibronectin in human fibrosarcoma cells possessing unique alpha and common beta subunits.
    J Cell Biol. 1987 Oct;105(4):1873-84 PMID: 2822727
  17. The pathogenesis of cancer metastasis.
    Nature. 1980 Jan 10;283(5743):139-46 PMID: 6985715
  18. Signal transduction through the fibronectin receptor induces collagenase and stromelysin gene expression.
    J Cell Biol. 1989 Aug;109(2):877-89 PMID: 2547805
  19. Isolation and characterization of Chinese hamster ovary cell variants deficient in the expression of fibronectin receptor.
    J Cell Biol. 1989 Dec;109(6 Pt 1):3157-67 PMID: 2531750
  20. The cellular basis of site-specific tumor metastasis.
    N Engl J Med. 1990 Mar 1;322(9):605-12 PMID: 2406604
  21. Tumor invasion and metastases--role of the extracellular matrix: Rhoads Memorial Award lecture.
    Cancer Res. 1986 Jan;46(1):1-7 PMID: 2998604
  22. Surface activation of the cell adhesion fragment of fibronectin.
    Exp Cell Res. 1984 Aug;153(2):550-5 PMID: 6734759
  23. Amino acid sequence of the human fibronectin receptor.
    J Cell Biol. 1987 Sep;105(3):1183-90 PMID: 2958481
  24. The integrin beta 1 subunit associates with the vitronectin receptor alpha v subunit to form a novel vitronectin receptor in a human embryonic kidney cell line.
    J Biol Chem. 1990 Apr 15;265(11):5938-41 PMID: 1690718
  25. The human leukocyte adhesion glycoprotein Mac-1 (complement receptor type 3, CD11b) alpha subunit. Cloning, primary structure, and relation to the integrins, von Willebrand factor and factor B.
    J Biol Chem. 1988 Sep 5;263(25):12403-11 PMID: 2457584
  26. Differential expression of a sialoglycoprotein with an approximate molecular weight of 900,000 on metastatic human colon carcinoma cells growing in culture and in tumor tissues.
    Cancer Res. 1988 May 1;48(9):2353-60 PMID: 3356003
  27. Alpha 2 beta 1 integrins from different cell types show different binding specificities.
    J Biol Chem. 1990 Jan 15;265(2):615-8 PMID: 2153105
  28. Analysis of fibronectin receptor function with monoclonal antibodies: roles in cell adhesion, migration, matrix assembly, and cytoskeletal organization.
    J Cell Biol. 1989 Aug;109(2):863-75 PMID: 2527241
  29. Cell surface receptors for extracellular matrix molecules.
    Annu Rev Cell Biol. 1987;3:179-205 PMID: 2825736
  30. Heterogeneous mutations in the beta subunit common to the LFA-1, Mac-1, and p150,95 glycoproteins cause leukocyte adhesion deficiency.
    Cell. 1987 Jul 17;50(2):193-202 PMID: 3594570
  31. A novel integrin beta subunit is associated with the vitronectin receptor alpha subunit (alpha v) in a human osteosarcoma cell line and is a substrate for protein kinase C.
    EMBO J. 1989 Oct;8(10):2955-65 PMID: 2479539
  32. The function of multiple extracellular matrix receptors in mediating cell adhesion to extracellular matrix: preparation of monoclonal antibodies to the fibronectin receptor that specifically inhibit cell adhesion to fibronectin and react with platelet glycoproteins Ic-IIa.
    J Cell Biol. 1988 Nov;107(5):1881-91 PMID: 2846588
  33. Identification and isolation of a 140 kd cell surface glycoprotein with properties expected of a fibronectin receptor.
    Cell. 1985 Jan;40(1):191-8 PMID: 3155652
  34. Human colon carcinoma cells use multiple receptors to adhere to laminin: involvement of alpha 6 beta 4 and alpha 2 beta 1 integrins.
    Cell Regul. 1990 Feb;1(3):249-57 PMID: 2100200
  35. Structure of integrin, a glycoprotein involved in the transmembrane linkage between fibronectin and actin.
    Cell. 1986 Jul 18;46(2):271-82 PMID: 3487386
  36. The platelet membrane glycoprotein IIb-IIIa complex.
    Blood. 1988 Apr;71(4):831-43 PMID: 2833328
  37. Absorptive and mucus-secreting subclones isolated from a multipotent intestinal cell line (HT-29) provide new models for cell polarity and terminal differentiation.
    J Cell Biol. 1987 Jul;105(1):345-57 PMID: 3611191
  38. The very late antigen family of heterodimers is part of a superfamily of molecules involved in adhesion and embryogenesis.
    Proc Natl Acad Sci U S A. 1987 May;84(10):3239-43 PMID: 3033641
  39. Analysis of integrin receptors for laminin and type IV collagen on metastatic B16 melanoma cells.
    Cancer Res. 1990 Feb 1;50(3):728-34 PMID: 2153445
  40. A rapid in vitro assay for quantitating the invasive potential of tumor cells.
    Cancer Res. 1987 Jun 15;47(12):3239-45 PMID: 2438036
  41. Involvement of LFA-1 in lymphoma invasion and metastasis demonstrated with LFA-1-deficient mutants.
    J Cell Biol. 1989 May;108(5):1979-85 PMID: 2654142
  42. Investigation of the biological effects of anti-cell adhesive synthetic peptides that inhibit experimental metastasis of B16-F10 murine melanoma cells.
    J Clin Invest. 1988 Mar;81(3):782-90 PMID: 3343338
  43. Cancer metastasis: tumor cell and host organ properties important in metastasis to specific secondary sites.
    Biochim Biophys Acta. 1988 Nov 15;948(2):175-224 PMID: 3052592
  44. Laminin receptor on platelets is the integrin VLA-6.
    Nature. 1988 Dec 1;336(6198):487-9 PMID: 2973567
  45. The role of cell adhesion proteins--laminin and fibronectin--in the movement of malignant and metastatic cells.
    Cancer Metastasis Rev. 1985;4(2):125-52 PMID: 3893683
  46. Expression of normal and mutant avian integrin subunits in rodent cells.
    J Cell Biol. 1989 Aug;109(2):853-61 PMID: 2788168
  47. Analysis of the tumor-associated antigen TSP-180. Identity with alpha 6-beta 4 in the integrin superfamily.
    J Biol Chem. 1989 Sep 15;264(26):15515-21 PMID: 2475502
  48. A novel vitronectin receptor integrin (alpha v beta x) is responsible for distinct adhesive properties of carcinoma cells.
    Cell. 1989 Apr 7;57(1):59-69 PMID: 2467745
  49. Phorbol ester modulation of integrin-mediated cell adhesion: a postreceptor event.
    J Cell Biol. 1989 May;108(5):1925-33 PMID: 2785521
  50. Synthetic peptide with cell attachment activity of fibronectin.
    Proc Natl Acad Sci U S A. 1983 Mar;80(5):1224-7 PMID: 6572380
  51. Cell matrix adhesion-related proteins VLA-1 and VLA-2: regulation of expression on T cells.
    J Immunol. 1987 May 1;138(9):2941-8 PMID: 3106495
  52. Inhibition of in vitro tumor cell invasion by Arg-Gly-Asp-containing synthetic peptides.
    J Cell Biol. 1988 Mar;106(3):925-30 PMID: 2450101
  53. Expression and function of a putative cell surface receptor for fibronectin in hamster and human cell lines.
    J Cell Biol. 1986 Oct;103(4):1595-603 PMID: 3021783
  54. Attachment, spreading and locomotion of avian neural crest cells are mediated by multiple adhesion sites on fibronectin molecules.
    EMBO J. 1988 Sep;7(9):2661-71 PMID: 3181137
  55. Association between fibronectin receptor and the substratum: spare receptors for cell adhesion.
    Exp Cell Res. 1987 Aug;171(2):376-88 PMID: 2957223
  56. Membrane receptors for extracellular matrix macromolecules: relationship to cell adhesion and tumor metastasis.
    Biochim Biophys Acta. 1987 Nov 25;907(3):261-78 PMID: 2445382
  57. Altered type I protein kinase in adhesion defective CHO cell variants.
    J Cell Physiol. 1987 Jan;130(1):118-24 PMID: 3027105
  58. Cell surface receptors for extracellular matrix components.
    Biochim Biophys Acta. 1990 Feb 28;1031(1):91-110 PMID: 1689589
Article Info
Journal
Clinical & experimental metastasis
Abbr.
Clin Exp Metastasis
ISSN
0262-0898
DOI
10.1007/BF01756387
Published
1991-00-00
Pages
163-78
Language
English
Region
Netherlands
NLM ID
8409970
Subset
IM
Grants
NCI NIH HHS · F32 CA 08614 · United States
NIGMS NIH HHS · GM 26165 · United States
External Links
PubMed source DOI: 10.1007/BF01756387 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2