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PMID: 3021783 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Expression and function of a putative cell surface receptor for fibronectin in hamster and human cell lines.

The Journal of cell biology ·Vol. 103 ·No. 4 ·1986-10-00 ·Pages 1595-603

Brown PJ, Juliano RL

Abstract

We have previously reported the use of monoclonal antibodies to identify a 140-kD cell surface glycoprotein in mammalian cells that is specifically involved in fibronectin-mediated cell adhesion. We now report the purification of this molecule using immunoaffinity chromatography and the subsequent generation of polyclonal antibodies that selectively immunoprecipitate 140-kD putative fibronectin receptor glycoprotein (gp140) extracted from rodent or human cells; these antibodies also specifically block fibronectin-mediated cell adhesion but not adhesion mediated by other factors in serum. Expression of gp140-like molecules was detected on the surfaces of several adherent human cell lines (HDF, WISH, and EFC) but not on erythrocytes; however, gp140 was also detected on a nonadherent human lymphoid line (DAUDI). Analysis of gp140 on nonreducing SDS gels revealed two closely migrating bands. Protease digestion and peptide mapping suggests that the two bands are closely related polypeptides.

MeSH Terms
Amnion Animals Antibodies, Monoclonal Antigens, Surface/physiology Cell Adhesion Cell Adhesion Molecules Cell Line Cricetinae Cricetulus Female Fibroblasts/analysis Fibronectins/physiology Glioblastoma Humans Lymphocytes Ovary Receptors, Fibronectin Receptors, Immunologic/analysis,physiology
Chemicals
Antibodies, Monoclonal Antigens, Surface Cell Adhesion Molecules Fibronectins Receptors, Fibronectin Receptors, Immunologic
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Brown P J
Juliano R L
References (33)
33 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. Characterization of a 140-kD avian cell surface antigen as a fibronectin-binding molecule.
    J Cell Biol. 1986 Feb;102(2):442-8 PMID: 2935540
  3. Protein antigens of normal and malignant human cells identified by immunoprecipitation with monoclonal antibodies.
    J Biol Chem. 1980 Jun 10;255(11):4980-3 PMID: 7372618
  4. Isolation and characterization of Chinese hamster ovary cell variants defective in adhesion to fibronectin-coated collagen.
    J Cell Biol. 1980 Dec;87(3 Pt 1):755-63 PMID: 7193214
  5. Membrane glycoproteins involved in cell--substratum adhesion.
    Proc Natl Acad Sci U S A. 1981 Oct;78(10):6071-5 PMID: 6273845
  6. Fibronectin-independent adhesion of fibroblasts to the extracellular matrix: mediation by a high molecular weight membrane glycoprotein.
    J Cell Biol. 1981 Dec;91(3 Pt 1):647-53 PMID: 7328114
  7. Fibronectins: multifunctional modular glycoproteins.
    J Cell Biol. 1982 Nov;95(2 Pt 1):369-77 PMID: 6128348
  8. A monoclonal antibody detaches embryonic skeletal muscle from extracellular matrices.
    J Cell Biol. 1982 Nov;95(2 Pt 1):654-66 PMID: 6183279
  9. Conformational states of fibronectin. Effects of pH, ionic strength, and collagen binding.
    J Biol Chem. 1982 Dec 25;257(24):14973-8 PMID: 7174679
  10. Inhibition of fibronectin receptor function by antibodies against baby hamster kidney cell wheat germ agglutinin receptors.
    J Cell Biol. 1982 Dec;95(3):876-84 PMID: 6296159
  11. Cell surface heparan sulfate mediates some adhesive responses to glycosaminoglycan-binding matrices, including fibronectin.
    J Cell Biol. 1983 Jan;96(1):112-23 PMID: 6219115
  12. Cell surface interactions with extracellular materials.
    Annu Rev Biochem. 1983;52:761-99 PMID: 6351729
  13. Isolation of human serum spreading factor.
    J Biol Chem. 1983 Oct 25;258(20):12548-52 PMID: 6630199
  14. Fibronectin-mediated binding and phagocytosis of polystyrene latex beads by baby hamster kidney cells.
    J Cell Biol. 1983 Nov;97(5 Pt 1):1515-23 PMID: 6630292
  15. Interaction of fibronectin-coated beads with CHO cells.
    Exp Cell Res. 1984 Jun;152(2):302-12 PMID: 6373325
  16. Surface activation of the cell adhesion fragment of fibronectin.
    Exp Cell Res. 1984 Aug;153(2):550-5 PMID: 6734759
  17. Dualistic nature of adhesive protein function: fibronectin and its biologically active peptide fragments can autoinhibit fibronectin function.
    J Cell Biol. 1984 Jul;99(1 Pt 1):29-36 PMID: 6736130
  18. An antibody that inhibits fibronectin-independent adhesion of fibroblasts to extracellular matrix material.
    J Cell Physiol. 1984 Sep;120(3):329-34 PMID: 6540267
  19. Localization of the gangliosides GD2 and GD3 in adhesion plaques and on the surface of human melanoma cells.
    Proc Natl Acad Sci U S A. 1984 Sep;81(18):5767-71 PMID: 6385004
  20. Variants of the cell recognition site of fibronectin that retain attachment-promoting activity.
    Proc Natl Acad Sci U S A. 1984 Oct;81(19):5985-8 PMID: 6237366
  21. Cleavage of a 135 kD cell surface glycoprotein correlates with loss of fibroblast adhesion to fibronectin.
    Exp Cell Res. 1985 Jan;156(1):182-90 PMID: 2981173
  22. Identification and isolation of a 140 kd cell surface glycoprotein with properties expected of a fibronectin receptor.
    Cell. 1985 Jan;40(1):191-8 PMID: 3155652
  23. A monoclonal antibody identifies a glycoprotein complex involved in cell-substratum adhesion.
    Exp Cell Res. 1985 Mar;157(1):218-26 PMID: 2578969
  24. The interaction of plasma fibronectin with fibroblastic cells in suspension.
    J Biol Chem. 1985 Apr 10;260(7):4492-500 PMID: 3920218
  25. Development of cell surface linkage complexes in cultured fibroblasts.
    J Cell Biol. 1985 Apr;100(4):1103-14 PMID: 3884631
  26. Selective inhibition of fibronectin-mediated cell adhesion by monoclonal antibodies to a cell-surface glycoprotein.
    Science. 1985 Jun 21;228(4706):1448-51 PMID: 4012302
  27. Synthetic peptides competitively inhibit both direct binding to fibroblasts and functional biological assays for the purified cell-binding domain of fibronectin.
    J Biol Chem. 1985 Sep 5;260(19):10402-5 PMID: 3161878
  28. A 125/115-kDa cell surface receptor specific for vitronectin interacts with the arginine-glycine-aspartic acid adhesion sequence derived from fibronectin.
    Proc Natl Acad Sci U S A. 1985 Sep;82(17):5766-70 PMID: 2412224
  29. Two distinct mechanisms for the interaction of cells with fibronectin substrata.
    J Cell Physiol. 1985 Jul;124(1):113-9 PMID: 3930514
  30. cAMP-induced phenotypic reversion of adhesion, aggregation, and endocytosis in adhesion-defective CHO cell variants.
    J Cell Physiol. 1985 Aug;124(2):337-43 PMID: 2413052
  31. Characterization of a membrane-associated glycoprotein complex implicated in cell adhesion to fibronectin.
    J Cell Biochem. 1985;28(4):307-18 PMID: 4055919
  32. The cell substrate attachment (CSAT) antigen has properties of a receptor for laminin and fibronectin.
    J Cell Biol. 1985 Dec;101(6):2134-44 PMID: 2933413
  33. Radioiodination of proteins in single polyacrylamide gel slices. Tryptic peptide analysis of all the major members of complex multicomponent systems using microgram quantities of total protein.
    J Biol Chem. 1977 Sep 25;252(18):6510-5 PMID: 893422
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.103.4.1595
Published
1986-10-00
Pages
1595-603
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2114351
Subset
IM
Grants
NIGMS NIH HHS · GM-26165 · United States
External Links
PubMed source DOI: 10.1083/jcb.103.4.1595 PMC Full Text View journal details
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