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PMID: 2654142 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Involvement of LFA-1 in lymphoma invasion and metastasis demonstrated with LFA-1-deficient mutants.

The Journal of cell biology ·Vol. 108 ·No. 5 ·1989-05-00 ·Pages 1979-85

Roossien FF, de Rijk D, Bikker A, Roos E

Abstract

Lymphocyte function-associated antigen-1 (LFA-1) is a leukocyte and lymphoma cell surface protein that promotes intercellular adhesion. We have previously shown that the invasion of hepatocyte cultures by lymphoma cells is inhibited by anti-LFA-1 antibodies (Roos, E., and F. F. Roossien. 1987. J. Cell Biol. 105:553-559). In addition, we now report that LFA-1 is also involved in invasion of lymphoma cells into fibroblast monolayers. To investigate the role of LFA-1 in metastasis of these lymphoma cells, we have generated mutants that are deficient in LFA-1 cell surface expression because of impaired synthesis of either the alpha or beta subunit precursor of LFA-1. We identified at least three distinct mutant clones. The invasive potential of the mutant cells in vitro, in both hepatocyte and fibroblast cultures, was considerably lower than that of parental cells. The metastatic potential of the mutants was much reduced, indicating that LFA-1 expression is required for efficient metastasis formation by certain lymphoma cells.

MeSH Terms
Animals Antigens, Differentiation/genetics,physiology Cell Adhesion Cells, Cultured Liver/cytology,pathology Lymphocyte Function-Associated Antigen-1 Lymphoma/genetics,pathology Membrane Glycoproteins/physiology Mice Mutation Neoplasm Invasiveness Neoplasm Metastasis
Chemicals
Antigens, Differentiation Lymphocyte Function-Associated Antigen-1 Membrane Glycoproteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Roossien F F
Division of Cell Biology, Antoni van Leeuwenhoekhuis, The Netherlands Cancer Institute, Amsterdam.
de Rijk D
Bikker A
Roos E
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.108.5.1979
Published
1989-05-00
Pages
1979-85
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2115560
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.108.5.1979 PMC Full Text View journal details
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