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PMID: 202928 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The sv40 transcription complex. I. Effect of viral chromatin proteins on endogenous RNA polymerase activity.

Nucleic acids research ·Vol. 4 ·No. 12 ·1977-12-00 ·Pages 4261-77

Brooks TL, Green MH

Abstract

SV40 chromatin obtained from infected monkey cells was used to study the effect of the viral chromatin proteins on endogenous RNA polymerase II. Ammonium sulfate activated the rate of transcription by endogenous RNA polymerase in two ways: 1) by direct action on the enzyme; and 2) by causing a reversible conformational change in the viral chromatin. Under optimal reaction conditions, the viral chromatin proteins did not limit the rate of RNA chain elongation, and high molecular weight RNA (1.6 X 10(6) d) was synthesized by the SV40 chromatin.

MeSH Terms
Cell Line Chromatin/metabolism DNA-Directed RNA Polymerases/metabolism Kinetics Molecular Weight Osmolar Concentration Simian virus 40/metabolism Transcription, Genetic Viral Proteins/metabolism
Chemicals
Chromatin Viral Proteins DNA-Directed RNA Polymerases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Brooks T L
Green M H
References (33)
33 references, click to expand
  1. Proteins in intracellular simian virus 40 nucleoportein complexes: comparison with simian virus 40 core proteins.
    J Virol. 1975 Mar;15(3):439-48 PMID: 163367
  2. Folding of the DNA double helix in chromatin-like structures from simian virus 40.
    Proc Natl Acad Sci U S A. 1975 May;72(5):1843-7 PMID: 168578
  3. Biogenesis and characterization of SV40 and polyoma RNAs in productively infected cells.
    Cold Spring Harb Symp Quant Biol. 1975;39 Pt 1:165-78 PMID: 169056
  4. Fingerprints of polyoma virus proteins and mouse histones.
    Cold Spring Harb Symp Quant Biol. 1975;39 Pt 1:235-41 PMID: 169064
  5. The organization of proteins in polyoma and cellular chromatin.
    Cold Spring Harb Symp Quant Biol. 1975;39 Pt 1:259-66 PMID: 169068
  6. Isolation and partial characterization of a nuclear RNA polymerase - SV40 DNA complex.
    FEBS Lett. 1975 Aug 1;56(1):149-55 PMID: 169150
  7. RNAs of simian virus 40 in productively infected monkey cells: kinetics of formation and decay in enucleate cells.
    Proc Natl Acad Sci U S A. 1975 Jul;72(7):2587-91 PMID: 170607
  8. Chromatin-like structures in polyoma virus and simian virus 10 lytic cycle.
    J Virol. 1975 Jan;17(1):204-11 PMID: 173884
  9. Isolation of two forms of SV40 nucleoprotein containing RNA polymerase from infected monkey cells.
    Virology. 1976 Jul 1;72(1):110-20 PMID: 180666
  10. Minichromosome of simian virus 40: presence of histone HI.
    Nucleic Acids Res. 1976 Aug;3(8):2101-13 PMID: 184438
  11. Properties of simian virus 40 transcriptional intermediates isolated from nuclei of permissive cells.
    J Virol. 1977 Jul;23(1):20-8 PMID: 196103
  12. Salt and divalent cations affect the flexible nature of the natural beaded chromatin structure.
    Nucleic Acids Res. 1977 Jun;4(6):1837-51 PMID: 197496
  13. RNA chain elongation on a chromatin template.
    Nucleic Acids Res. 1976 Sep;3(9):2223-31 PMID: 787933
  14. Salt-induced structural changes in nucleosomes.
    Nucleic Acids Res. 1977 Apr;4(4):853-66 PMID: 866195
  15. Transcription of DNA and chromatin with calf thymus RNA polymerase B in vitro.
    J Mol Biol. 1975 Jun 25;95(2):257-69 PMID: 1102705
  16. Electron microscopic and biochemical evidence that chromatin structure is a repeating unit.
    Cell. 1975 Apr;4(4):281-300 PMID: 1122558
  17. Spheroid chromatin units (v bodies).
    Science. 1974 Jan 25;183(4122):330-2 PMID: 4128918
  18. Selective extraction of polyoma DNA from infected mouse cell cultures.
    J Mol Biol. 1967 Jun 14;26(2):365-9 PMID: 4291934
  19. Isolation of a polyoma-nucleoprotein complex from infected mouse-cell cultures.
    Proc Natl Acad Sci U S A. 1971 May;68(5):1032-6 PMID: 4324998
  20. Nucleoprotein complexes in simian virus 40-infected cells.
    J Virol. 1971 Oct;8(4):363-71 PMID: 4331646
  21. Genetic analysis of simian virus 40. 3. Characterization of a temperature-sensitive mutant blocked at an early stage of productive infection in monkey cells.
    J Virol. 1972 Jun;9(6):956-68 PMID: 4338641
  22. Properties of nucleoprotein complexes containing replicating polyoma DNA.
    J Virol. 1973 Oct;12(4):887-900 PMID: 4359957
  23. Nucleoprotein complexes containing replicating Simian virus 40 DNA: comparison with polyoma nucleoprotein complexes.
    J Virol. 1973 Oct;12(4):901-8 PMID: 4359958
  24. Animal DNA-dependent RNA polymerases. Analysis of the RNAs synthesized on Simian virus 40 superhelical DNA by mammalian RNA polymerases AI and B.
    Eur J Biochem. 1974 Jan 16;41(2):379-95 PMID: 4361418
  25. Animal DNA-dependent RNA polymerases. Studies on the binding of mammalian RNA polymerases AI and B to Simian virus 40 DNA.
    Eur J Biochem. 1974 Jan 16;41(2):397-411 PMID: 4361419
  26. Properties of the polyoma virus transcription complex obtained from mouse nuclei.
    Virology. 1974 Jan;57(1):122-7 PMID: 4362020
  27. Transcription of the early region of bacteriophage T7: selective initiation with dinucleotides.
    J Mol Biol. 1973 Jun 25;77(2):255-77 PMID: 4587752
  28. Transcription during bacteriophage T4 development: synthesis and relative stability of early and late RNA.
    J Mol Biol. 1968 Feb 14;31(3):325-48 PMID: 4866329
  29. Studies on the role of ammonium sulfate in nuclear transcription in vitro.
    Biochim Biophys Acta. 1972 Jun 22;272(1):119-23 PMID: 5043708
  30. Transcription of mammalian chromatin by mammalian DNA-dependent RNA polymerases.
    Eur J Biochem. 1971 Nov 11;23(2):229-41 PMID: 5156371
  31. A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells.
    Proc Natl Acad Sci U S A. 1967 May;57(5):1514-21 PMID: 5231757
  32. The Biology of Isolated Chromatin: Chromosomes, biologically active in the test tube, provide a powerful tool for the study of gene action.
    Science. 1968 Jan 5;159(3810):47-56 PMID: 17737465
  33. Chromatin structure: deduced from a minichromosome.
    Science. 1975 Mar 28;187(4182):1202-3 PMID: 17754289
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/4.12.4261
Published
1977-12-00
Pages
4261-77
Language
English
Region
England
NLM ID
0411011
PMCID
PMC343239
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/4.12.4261 PMC Full Text View journal details
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