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PMID: 866195 Published · ppublish English Journal Article

Salt-induced structural changes in nucleosomes.

Nucleic acids research ·Vol. 4 ·No. 4 ·1977-04-00 ·Pages 853-66

Sahasrabuddhe CG, Saunders GF

Abstract

Nucleosomes and oligonucleosomes were prepared by digestion of human placental nuclei with staphlococcal nuclease and fractionated by gel filtration chromatography. The effect of increasing salt on the structure of nucleosomes was examined in the presence and absence of 10 mM MgCl2. Nucleosomes and oligonucleosomes are insoluble over a broad range of salt concentration. Nucleosomes are insoluble in larger than or equal to 120 mM (NH4)2SO4 containing 10 mM MgCl2 allowing analyses of changes in nucleosomal DNA by C.D. spectroscopy. Nucleosomes are insoluble in less than or equal to 120 mM (NH4)2SO4 containing 10 mM MgCl2 as demonstrated by turbidity measurements. We conclude that the insolubility of nucleosomes accompanies salt-induced structural changes possibly due to individual particle condensation. As the salt concentration is increased the nucleosomes condense and then relax at higher salt concentrations.

MeSH Terms
Ammonium Sulfate Cell Nucleus/analysis Chemical Phenomena Chemistry Chromatin/analysis Circular Dichroism DNA Female Humans Macromolecular Substances Magnesium Micrococcal Nuclease Osmolar Concentration Placenta/analysis Pregnancy Solubility
Chemicals
Chromatin Macromolecular Substances DNA Micrococcal Nuclease Magnesium Ammonium Sulfate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sahasrabuddhe C G
Saunders G F
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37 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/4.4.853
Published
1977-04-00
Pages
853-66
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342489
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/4.4.853 PMC Full Text View journal details
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