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PMID: 1052536 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Low-angle neutron scattering from chromatin subunit particles.

Nucleic acids research ·Vol. 2 ·No. 11 ·1975-11-00 ·Pages 2163-76

Pardon JF, Worcester DL, Wooley JC, Tatchell K, Van Holde KE, Richards BM

Abstract

Monomer chromatin particles containing 140 base pairs of DNA and eight histone molecules have been studied by neutron scattering. From measurements in various H2O/D2O mixtures, radii of gyration and the average scattering density of the particle were determined. The radius of gyration under conditions when scattering from the DNA dominates is 50A, and when scattering from the protein dominates, 30A. Consequently the core of the particle is largely occupied by the histones while the outer shell consists of DNA together with some of the histone.

MeSH Terms

Animals Chickens Chromatin/ultrastructure Erythrocytes/ultrastructure Mathematics Models, Structural Neutrons Scattering, Radiation

Chemicals

Chromatin

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Pardon J F

Worcester D L

Wooley J C

Tatchell K

Van Holde K E

Richards B M

References (18)

18 references, click to expand

Comparison of neutron and X-ray scattering of dilute myoglobin solutions.
J Mol Biol. 1975 Apr 5;93(2):255-65 PMID: 1171250
Spheroid chromatin units (v bodies).
Science. 1974 Jan 25;183(4122):330-2 PMID: 4128918
The effect of deuterium ion concentration on the properties of sarcoplasmic reticulum.
J Membr Biol. 1974;17(2):189-97 PMID: 4276282
Asymmetry in the 50S ribosomal subunit of Escherichia coli.
Proc Natl Acad Sci U S A. 1974 Jan;71(1):172-6 PMID: 4589891
Electron microscopy of chromatin subunit particles.
Biochem Biophys Res Commun. 1974 Oct 23;60(4):1365-70 PMID: 4473144
Chromatographic separation of chromatin subunits.
Biochem Biophys Res Commun. 1974 Dec 23;61(4):1193-8 PMID: 4455255
Subunit structure of chromatin.
Nature. 1974 Sep 20;251(5472):249-51 PMID: 4422492
The effect of trypsin on nuclease-resistant chromatin fragments.
J Biol Chem. 1974 Jan 10;249(1):152-6 PMID: 4855624
Chromatin structure: a repeating unit of histones and DNA.
Science. 1974 May 24;184(4139):868-71 PMID: 4825889
Chromatin sub-structure. The digestion of chromatin DNA at regularly spaced sites by a nuclear deoxyribonuclease.
Biochem Biophys Res Commun. 1973 May 15;52(2):504-10 PMID: 4711166
A neutron scattering study of the distribution of protein and RNA in the 30 S ribosomal subunit of Escherichia coli.
J Mol Biol. 1975 Jan 5;91(1):101-20 PMID: 1102695
Chromatin architecture: investigation of a subunit of chromatin by dark field electron microscopy.
Proc Natl Acad Sci U S A. 1975 Jul;72(7):2691-5 PMID: 1058483
Cleavage of DNA in nuclei and chromatin with staphylococcal nuclease.
Biochemistry. 1975 Jul;14(13):2921-5 PMID: 1148185
A comparison of the digestion of nuclei and chromatin by staphylococcal nuclease.
Biochemistry. 1975 Jul;14(13):2915-20 PMID: 1148184
The subunit structure of the eukaryotic chromosome.
Nature. 1975 Jan 24;253(5489):245-9 PMID: 1167623
Electron microscopic and biochemical evidence that chromatin structure is a repeating unit.
Cell. 1975 Apr;4(4):281-300 PMID: 1122558
A model for particulate structure in chromatin.
Nucleic Acids Res. 1974 Nov;1(11):1579-86 PMID: 10793713
Internal structure of the chromatin subunit.
Nucleic Acids Res. 1974 Nov;1(11):1573-8 PMID: 10793712

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/2.11.2163

Published

1975-11-00

Pages

2163-76

Language

English

Region

England

NLM ID

0411011

PMCID

PMC343581

Subset

External Links

PubMed source DOI: 10.1093/nar/2.11.2163 PMC Full Text View journal details

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Low-angle neutron scattering from chromatin subunit particles.

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