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PMID: 2027755 Published · ppublish English Journal Article

Non-methylated islands in fish genomes are GC-poor.

Nucleic acids research ·Vol. 19 ·No. 7 ·1991-04-11 ·Pages 1469-74

Cross S, Kovarik P, Schmidtke J, Bird A

Abstract

In the vertebrate genomes studied to date the 5' end of many genes are associated with distinctive sequences known as CpG islands. CpG islands have three properties: they are non-methylated; the dinucleotide CpG occurs at the frequency predicted by base composition; and they are GC-rich. Unexpectedly we have found that CpG islands in certain fish only have the first two properties; that is, their GC-content is not elevated compared to bulk genomic DNA. Based on this finding, we speculate that the GC-richness of CpG islands in vertebrates other than fish is a passive consequence of a higher mutation rate in regions of open chromatin under conditions where the nucleotide precursor pools are biased.

MeSH Terms
Actins/genetics,metabolism Animals Base Sequence Blotting, Southern Cytosine/analysis DNA/analysis Dinucleoside Phosphates/metabolism Drosophila/genetics Fishes/genetics Guanine/analysis Humans Metallothionein/genetics,metabolism Methylation Molecular Sequence Data Rats
Chemicals
Actins Dinucleoside Phosphates cytidylyl-3'-5'-guanosine Guanine Cytosine DNA Metallothionein
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Cross S
Research Institute for Molecular Pathology, Vienna, Austria.
Kovarik P
Schmidtke J
Bird A
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/19.7.1469
Published
1991-04-11
Pages
1469-74
Language
English
Region
England
NLM ID
0411011
PMCID
PMC333903
Subset
IM
Databases
GENBANK
M24113
External Links
PubMed source DOI: 10.1093/nar/19.7.1469 PMC Full Text View journal details
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