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PMID: 2027748 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Hypersensitive site 4 of the human beta globin locus control region.

Nucleic acids research ·Vol. 19 ·No. 7 ·1991-04-11 ·Pages 1413-9

Pruzina S, Hanscombe O, Whyatt D, Grosveld F, Philipsen S

Abstract

The Locus Control Region (LCR) of the human beta globin gene domain is defined by four erythroid-specific DNasel hypersensitive sites (HSS) located upstream of this multigene cluster. The LCR confers copy number dependent high levels of erythroid specific expression to a linked transgene, independent of the site of integration. To assess the role of the individual hypersensitive sites of the LCR, we have localized HSS4 to a 280bp fragment that is functional both in murine erythroleukaemia (MEL) cells and in transgenic mice. This fragment coincides with the major area of hypersensitivity 'in vivo' and contains a number of DNasel footprints. Bandshift analysis shows that these footprints correspond to binding sites for the erythroid specific proteins GATA1 and NF-E2 and a number of ubiquitous proteins, including jun/fos, Sp1 and TEF2.

MeSH Terms
Animals Base Sequence Binding Sites Blotting, Southern Chromosome Mapping DNA/analysis DNA Fingerprinting Deoxyribonuclease I/metabolism Electrophoresis, Agar Gel Globins/genetics HeLa Cells Humans Mice Mice, Transgenic Molecular Sequence Data Regulatory Sequences, Nucleic Acid
Chemicals
Globins DNA Deoxyribonuclease I
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Pruzina S
National Institute for Medical Research, Mill Hill, London, UK.
Hanscombe O
Whyatt D
Grosveld F
Philipsen S
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33 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/19.7.1413
Published
1991-04-11
Pages
1413-9
Language
English
Region
England
NLM ID
0411011
PMCID
PMC333894
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/19.7.1413 PMC Full Text View journal details
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