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PMID: 2026653 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Molecular cloning and characterization of B-cadherin, a novel chick cadherin.

The Journal of cell biology ·Vol. 113 ·No. 4 ·1991-05-00 ·Pages 893-905

Napolitano EW, Venstrom K, Wheeler EF, Reichardt LF

Abstract

Calcium-dependent cell-cell adhesion is mediated in large part by a set of homologous integral membrane glycoproteins termed cadherins. In this report, antibodies to conserved domains in previously described cadherins have been used to isolate cDNAs encoding a novel chick cadherin. The deduced primary structure of this novel molecule, assigned the name B-cadherin, contains 726 amino acid residues which include five extracellular domains characteristic of this class of adhesion molecules, a single putative transmembrane spanning region, and a cytoplasmic tail. In each domain, B-cadherin shares extensive homologies with other cadherins, but is more closely related to E-cadherin, P-cadherin, and L-CAM than to N-cadherin. It is expressed in a wide variety of chick tissues at embryonic day 13. In particular, immunohistochemical staining and in situ hybridization localize B-cadherin protein and mRNA to the epithelial lining of the choroid plexus and to cells in specific layers of the optic tectum in chick brain. Levels of the protein and RNA transcript change dramatically as development proceeds in chick brain. These results suggest that B-cadherin has important functions in neurogenesis, in at least some epithelia, and in embryogenesis.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Blotting, Northern Blotting, Western Brain Cadherins/genetics,immunology Chick Embryo Chickens Cloning, Molecular DNA/genetics Gene Expression Molecular Sequence Data Nucleic Acid Hybridization RNA, Messenger/genetics
Chemicals
Cadherins RNA, Messenger DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Napolitano E W
Department of Physiology, University of California, San Francisco, 94143-0724.
Venstrom K
Wheeler E F
Reichardt L F
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.113.4.893
Published
1991-05-00
Pages
893-905
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2288997
Subset
IM
Grants
NHLBI NIH HHS · HL43397 · United States
Databases
GENBANK
58518, M60691, M60692, M60693, M60694, M60695, M60696, M60697, X56698, X56699, X58518
External Links
PubMed source DOI: 10.1083/jcb.113.4.893 PMC Full Text View journal details
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