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PMID: 2390969 Published · ppublish English Comparative Study Journal Article

Identification and cloning of two species of cadherins in bovine endothelial cells.

The EMBO journal ·Vol. 9 ·No. 9 ·1990-09-00 ·Pages 2701-8

Liaw CW, Cannon C, Power MD, Kiboneka PK, Rubin LL

Abstract

By taking advantage of the extensive homology found in the cytoplasmic domains of several cloned cadherin molecules, we were able to identify two species of cadherins in bovine aortic endothelial cells using the polymerase chain reaction (PCR). The two species of PCR products were subsequently used as DNA probes to isolate the corresponding cDNA clones from bovine adrenal microvascular endothelial cells. Sequence comparison with other characterized cadherin molecules indicates that the major cDNA species encodes a cadherin molecule highly homologous to chicken and mouse N-cadherins, while the minor species is most homologous to mouse and human P-cadherins. Northern blot analysis with the corresponding cDNA probe showed a wide distribution of bovine N-cadherin among non-neuronal, as well as neuronal tissues, while P-cadherin was most abundant in kidney among all the bovine tissues tested, but was undetectable in placenta.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Capillaries/metabolism Cattle Cell Adhesion Molecules/genetics Cell Line Cells, Cultured Cerebrovascular Circulation Cloning, Molecular DNA/genetics DNA Probes Endothelium, Vascular/metabolism Immunoblotting Molecular Sequence Data Organ Specificity Polymerase Chain Reaction Restriction Mapping Sequence Homology, Nucleic Acid
Chemicals
Cell Adhesion Molecules DNA Probes DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Liaw C W
Athena Neurosciences, Inc., South San Francisco, CA 94080.
Cannon C
Power M D
Kiboneka P K
Rubin L L
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-09-00
Pages
2701-8
Language
English
Region
England
NLM ID
8208664
PMCID
PMC551975
Subset
IM
Databases
GENBANK
X53614, X53615
External Links
PubMed source PMC Full Text View journal details
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