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PMID: 20144757 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Methods in mammalian autophagy research.

Cell ·Vol. 140 ·No. 3 ·2010-02-05 ·Pages 313-26

Mizushima N, Yoshimori T, Levine B

Abstract

Autophagy has been implicated in many physiological and pathological processes. Accordingly, there is a growing scientific need to accurately identify, quantify, and manipulate the process of autophagy. However, as autophagy involves dynamic and complicated processes, it is often analyzed incorrectly. In this Primer, we discuss methods to monitor autophagy and to modulate autophagic activity, with a primary focus on mammalian macroautophagy.

MeSH Terms
Animals Autophagy Cytological Techniques Humans Phagosomes/metabolism
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Mizushima Noboru
Department of Physiology and Cell Biology, Tokyo Medical and Dental University, Tokyo 113-8519, Japan. nmizu.phy2@tmd.ac.jp
Yoshimori Tamotsu
Levine Beth
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Article Info
Journal
Cell
Abbr.
Cell
ISSN
1097-4172
DOI
10.1016/j.cell.2010.01.028
Email
nmizu.phy2@tmd.ac.jp
Published
2010-02-05
Pages
313-26
Language
English
Region
United States
NLM ID
0413066
PMCID
PMC2852113
Subset
IM
Grants
NCI NIH HHS · R01 CA084254-10S1 · United States
NIAID NIH HHS · U54 AI057156 · United States
NCI NIH HHS · R01 CA084254-10 · United States
NCI NIH HHS · R01 CA084254 · United States
NIAID NIH HHS · U54 AI057156-06S18820 · United States
NCI NIH HHS · R01 CA85254 · United States
NCI NIH HHS · R01 CA109618-05 · United States
NCI NIH HHS · R01 CA109618 · United States
External Links
PubMed source DOI: 10.1016/j.cell.2010.01.028 PMC Full Text View journal details
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