Abstract
RNA virus infection is recognized by retinoic acid-inducible gene (RIG)-I-like receptors (RLRs), RIG-I, and melanoma differentiation-associated gene 5 (MDA5) in the cytoplasm. RLRs are comprised of N-terminal caspase-recruitment domains (CARDs) and a DExD/H-box helicase domain. The third member of the RLR family, LGP2, lacks any CARDs and was originally identified as a negative regulator of RLR signaling. In the present study, we generated mice lacking LGP2 and found that LGP2 was required for RIG-I- and MDA5-mediated antiviral responses. In particular, LGP2 was essential for type I IFN production in response to picornaviridae infection. Overexpression of the CARDs from RIG-I and MDA5 in Lgp2(-/-) fibroblasts activated the IFN-beta promoter, suggesting that LGP2 acts upstream of RIG-I and MDA5. We further examined the role of the LGP2 helicase domain by generating mice harboring a point mutation of Lys-30 to Ala (Lgp2 (K30A/K30A)) that abrogated the LGP2 ATPase activity. Lgp2 (K30A/K30A) dendritic cells showed impaired IFN-beta productions in response to various RNA viruses to extents similar to those of Lgp2(-/-) cells. Lgp2(-/-) and Lgp2 (K30A/K30A) mice were highly susceptible to encephalomyocarditis virus infection. Nevertheless, LGP2 and its ATPase activity were dispensable for the responses to synthetic RNA ligands for MDA5 and RIG-I. Taken together, the present data suggest that LGP2 facilitates viral RNA recognition by RIG-I and MDA5 through its ATPase domain.
MeSH Terms
Animals
Cardiovirus Infections/genetics,immunology
Cell Line
DEAD Box Protein 58
DEAD-box RNA Helicases/immunology,metabolism
Encephalomyocarditis virus/immunology
Female
Interferon-Induced Helicase, IFIH1
Interferon-beta/biosynthesis,genetics,immunology
Mice
Mice, Inbred C57BL
Mice, Knockout
RNA Helicases/deficiency,genetics,immunology,metabolism
RNA, Viral/immunology
Signal Transduction
Survival Rate
Chemicals
RNA, Viral
Interferon-beta
Dhx58 protein, mouse
Ddx58 protein, mouse
Ifih1 protein, mouse
DEAD Box Protein 58
DEAD-box RNA Helicases
Interferon-Induced Helicase, IFIH1
RNA Helicases
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Satoh Takashi
Laboratory of Host Defense, WPI Immunology Frontier Research Center, Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan.
Kato Hiroki
Kumagai Yutaro
Yoneyama Mitsutoshi
Sato Shintaro
Matsushita Kazufumi
Tsujimura Tohru
Fujita Takashi
Akira Shizuo
Takeuchi Osamu
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