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PMID: 11489944 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Transplanted long-term cultured pre-BI cells expressing calpastatin are resistant to B cell receptor-induced apoptosis.

The Journal of experimental medicine ·Vol. 194 ·No. 3 ·2001-08-06 ·Pages 247-54

Ruiz-Vela A, Serrano F, González MA, Abad JL, Bernad A, Maki M, Martínez-A C

Abstract

Long-term cultured pre-B cells are able to differentiate into immunoglobulin (Ig)M-positive B cells (IgM(+) cells) when transplanted into severe combined immunodeficient (SCID) mice. Based on previous studies, here we report the development of a reconstitution assay in nonobese diabetic/SCID (NOD/SCID) mice using pre-B cells, which allows us to study the role of calpains (calcium-activated endopeptidases) during B cell development as well as in B cell clonal deletion. Using this model, we show that calpastatin (the natural inhibitor of calpains) inhibits B cell receptor-induced apoptosis in IgM(+) cells derived from transplanted mice. We thus hypothesize an important function for calpain in sculpting the B cell repertoire.

MeSH Terms
Animals Apoptosis/immunology B-Lymphocytes/cytology,immunology,metabolism,transplantation Calcium-Binding Proteins/genetics,metabolism Calpain/metabolism Cell Differentiation Cell Line Clonal Deletion Gene Expression Hematopoiesis Hematopoietic Stem Cell Transplantation Hematopoietic Stem Cells/cytology,immunology,metabolism Immunoglobulin M/metabolism Interleukin-7/metabolism Mice Mice, Inbred NOD Mice, SCID Models, Biological Receptors, Antigen, B-Cell/metabolism
Chemicals
Calcium-Binding Proteins Immunoglobulin M Interleukin-7 Receptors, Antigen, B-Cell calpastatin Calpain
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Ruiz-Vela A
Department of Immunology and Oncology, Centro Nacional de Biotecnología/CSIC, Universidad Autónoma de Madrid, Campus de Cantoblanco UAM, E-28049 Madrid, Spain. aruiz@cnb.uam.es
Serrano F
González M A
Abad J L
Bernad A
Maki M
Martínez-A C
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.194.3.247
Email
aruiz@cnb.uam.es
Published
2001-08-06
Pages
247-54
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2193469
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.194.3.247 PMC Full Text View journal details
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