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PMID: 20023653 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

A robust and high-throughput Cre reporting and characterization system for the whole mouse brain.

Nature neuroscience ·Vol. 13 ·No. 1 ·2010-01-00 ·Pages 133-40

Madisen L, Zwingman TA, Sunkin SM, Oh SW, Zariwala HA, Gu H, Ng LL, Palmiter RD, Hawrylycz MJ, Jones AR, Lein ES, Zeng H

Abstract

The Cre/lox system is widely used in mice to achieve cell-type-specific gene expression. However, a strong and universally responding system to express genes under Cre control is still lacking. We have generated a set of Cre reporter mice with strong, ubiquitous expression of fluorescent proteins of different spectra. The robust native fluorescence of these reporters enables direct visualization of fine dendritic structures and axonal projections of the labeled neurons, which is useful in mapping neuronal circuitry, imaging and tracking specific cell populations in vivo. Using these reporters and a high-throughput in situ hybridization platform, we are systematically profiling Cre-directed gene expression throughout the mouse brain in several Cre-driver lines, including new Cre lines targeting different cell types in the cortex. Our expression data are displayed in a public online database to help researchers assess the utility of various Cre-driver lines for cell-type-specific genetic manipulation.

MeSH Terms
Animals Bacterial Proteins/genetics Brain/cytology,metabolism Dendrites/metabolism Gene Transfer Techniques Integrases/genetics,physiology Luminescent Proteins/genetics Mice Mice, Inbred C57BL Mice, Transgenic Microscopy, Confocal Neurons/cytology Promoter Regions, Genetic Recombination, Genetic Regulatory Sequences, Nucleic Acid Tamoxifen/pharmacology Tissue Distribution Viral Proteins/genetics,metabolism
Chemicals
Bacterial Proteins Luminescent Proteins Viral Proteins yellow fluorescent protein, Bacteria Tamoxifen Cre recombinase Integrases
Authors & Affiliations
12 authors, click to expand affiliations / ORCID
Madisen Linda
Allen Institute for Brain Science, Seattle, Washington, USA.
Zwingman Theresa A
Sunkin Susan M
Oh Seung Wook
Zariwala Hatim A
Gu Hong
Ng Lydia L
Palmiter Richard D
Hawrylycz Michael J
Jones Allan R
Lein Ed S
Zeng Hongkui
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Article Info
Journal
Nature neuroscience
Abbr.
Nat Neurosci
ISSN
1546-1726
DOI
10.1038/nn.2467
Published
2010-01-00
Epub
2009-00-20
Pages
133-40
Language
English
Region
United States
NLM ID
9809671
PMCID
PMC2840225
Subset
IM
Grants
NIMH NIH HHS · R01 MH085500 · United States
NIMH NIH HHS · R01 MH085500-01 · United States
NIMH NIH HHS · MH085500 · United States
External Links
PubMed source DOI: 10.1038/nn.2467 PMC Full Text View journal details
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