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PMID: 20018620 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Gene expression analysis of macrophages that facilitate tumor invasion supports a role for Wnt-signaling in mediating their activity in primary mammary tumors.

Journal of immunology (Baltimore, Md. : 1950) ·Vol. 184 ·No. 2 ·2010-01-15 ·Pages 702-12

Ojalvo LS, Whittaker CA, Condeelis JS, Pollard JW

Abstract

The tumor microenvironment modifies the malignancy of tumors. In solid tumors, this environment is populated by many macrophages that, in genetic studies that depleted these cells from mouse models of breast cancer, were shown to promote tumor progression to malignancy and increase metastatic potential. Mechanistic studies showed that these tumor-promoting effects of macrophages are through the stimulation of tumor cell migration, invasion, intravasation, and enhancement of angiogenesis. Using an in vivo invasion assay, it was demonstrated that invasive carcinoma cells are a unique subpopulation of tumor cells whose invasion and chemotaxis is dependent on the comigration of tumor-associated macrophages (TAMs) with obligate reciprocal signaling through an epidermal growth factor-CSF-1 paracrine loop. In this study, these invasion-promoting macrophages were isolated and subjected to analysis of their transcriptome in comparison with TAMs isolated indiscriminately to function using established macrophage markers. Unsupervised analysis of transcript patterns showed that the invasion-associated TAMs represent a unique subpopulation of TAMs that, by gene ontology criteria, have gene expression patterns related to tissue and organ development. Gene set enrichment analysis showed that these macrophages are also specifically enriched for molecules involved in Wnt-signaling. Previously, it was shown that macrophage-derived Wnt molecules promote vascular remodeling and that tumor cells are highly motile and intravasate around perivascular TAM clusters. Taken together, we conjecture that invasive TAMs link angiogenesis and tumor invasion and that Wnt-signaling plays a role in mediating their activity.

MeSH Terms
Animals Cell Movement Epidermal Growth Factor Gene Expression Profiling Macrophages/metabolism Mammary Neoplasms, Animal/immunology,pathology Mice Neoplasm Invasiveness/genetics,immunology Neovascularization, Pathologic Paracrine Communication RNA, Messenger/analysis Signal Transduction Wnt Proteins/metabolism
Chemicals
RNA, Messenger Wnt Proteins Epidermal Growth Factor
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ojalvo Laureen S
Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
Whittaker Charles A
Condeelis John S
Pollard Jeffrey W
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Article Info
Journal
Journal of immunology (Baltimore, Md. : 1950)
Abbr.
J Immunol
ISSN
1550-6606
Published
2010-01-15
Epub
2009-00-16
Pages
702-12
Language
English
Region
United States
NLM ID
2985117R
PMCID
PMC3226722
Subset
IM
Grants
NCI NIH HHS · P01 CA100324 · United States
NCI NIH HHS · CA100324 · United States
NCI NIH HHS · R01 CA131270-05 · United States
PHS HHS · P30 133330 · United States
NCI NIH HHS · P30 CA014051 · United States
NCI NIH HHS · CA131270 · United States
NCI NIH HHS · P30-CA14051 · United States
NIGMS NIH HHS · T32 GM 07288 · United States
NCI NIH HHS · P01 CA100324-09 · United States
NCI NIH HHS · U54 CA126511-05 · United States
NIGMS NIH HHS · T32 GM007288 · United States
NCI NIH HHS · CA126511 · United States
NCI NIH HHS · U54 CA126511 · United States
NCI NIH HHS · R01 CA131270 · United States
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