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PMID: 2000085 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Upstream sequences regulating legumin gene expression in heterologous transgenic plants.

Molecular & general genetics : MGG ·Vol. 225 ·No. 1 ·1991-01-00 ·Pages 121-8

Bäumlein H, Boerjan W, Nagy I, Panitz R, Inzé D, Wobus U

Abstract

We have previously isolated a legumin gene LeB4 from Vicia faba and shown that a 4.7 kb DNA fragment containing the gene leads to seed-specific expression in transgenic tobacco plants. Here we report that the 2.4 kb upstream sequence alone, when fused to either the neomycin phosphotransferase II (nptII) gene or the beta-glucuronidase (uidA) gene, leads to high enzyme levels in transgenic seeds of both tobacco and Arabidopsis. beta-Glucuronidase (GUS) activity is especially intense in the cotyledons fading out towards the embryonal root tip, a result confirmed by in situ hybridization. Staining of endosperm cells is consistent in both species. Analysis of a series of promoter deletion mutants fused to the nptII gene and introduced into tobacco plants revealed that about 1 kb of 5'-flanking sequence is sufficient for high-level expression but indirect evidence suggests the presence of weak positive regulatory elements further upstream. Deletions leaving only 0.2 kb of upstream sequence reduce enzyme levels to less than 10%. A deletion which destroys the legumin box with its seed protein gene-specific CATGCATG motif has no obvious effects on expression levels.

MeSH Terms
Blotting, Southern Cloning, Molecular Gene Expression Regulation Nucleic Acid Hybridization Organ Specificity/genetics Plant Proteins Plant Proteins, Dietary/genetics Plants/genetics Plants, Toxic Promoter Regions, Genetic Regulatory Sequences, Nucleic Acid Tobacco/genetics
Chemicals
Plant Proteins Plant Proteins, Dietary legumin protein, plant
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Bäumlein H
Zentralinstitut für Genetik und Kulturpflanzenforschung, Akademie der Wisssenschaften, Gatersleben, Federal Republic of Germany.
Boerjan W
Nagy I
Panitz R
Inzé D
Wobus U
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28 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00282650
Published
1991-01-00
Pages
121-8
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00282650
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