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PMID: 16593906 Published · ppublish English Journal Article

Cellular localization of soybean storage protein mRNA in transformed tobacco seeds.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 2 ·1988-01-00 ·Pages 458-62

Barker SJ, Harada JJ, Goldberg RB

Abstract

We transformed tobacco plants with a soybean beta-conglycinin gene that encodes the 1.7-kilobase beta-subunit mRNA. We showed that the beta-conglycinin mRNA accumulates and decays during tobacco seed development and that beta-conglycinin mRNA is undetectable in the tobacco leaf. We utilized in situ hybridization to localize beta-conglycinin mRNA within the tobacco seed. beta-Conglycinin mRNA is not detectable within the endosperm but is localized within specific embryonic cell types. The highest concentration of beta-conglycinin mRNA is found in cotyledon storage parenchyma cells. We conclude that sequences required for embryo expression, temporal control, and cell specificity are linked to the beta-conglycinin gene, and that factors regulating beta-conglycinin gene expression are compartmentalized within analogous soybean and tobacco seed regions.

Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Barker S J
Department of Biology, University of California, Los Angeles, CA 90024.
Harada J J
Goldberg R B
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.2.458
Published
1988-01-00
Pages
458-62
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC279569
External Links
PubMed source DOI: 10.1073/pnas.85.2.458 PMC Full Text View journal details
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