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PMID: 198782 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Mechanism of translational control by hemin in reticulocyte lysates.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 8 ·1977-08-00 ·Pages 3326-9

Datta A, de Haro C, Sierra JM, Ochoa S

Abstract

The formation of translational inhibitor (active eIF-2 kinase) from proinhibitor (inactive eIF-2 kinase) in reticulocyte lysates, known to be controlled by hemin, can, as we recently reported, be induced by 3':5'-cyclic AMP(cAMP)-dependent protein kinase (ATP:protein phosphotransferase, EC 2.7.1.37) or its catalytic subunit. We find that in crude preparations from rabbit reticulocyte lysates, hemin inhibits the conversion of proinhibitor to inhibitor catalyzed by endogenous cAMP-dependent protein kinase upon addition of cAMP, but not that caused by the addition of free protein kinase catalytic subunit. Hemin prevents the binding of cAMP to the regulatory subunit of cAMP-dependent protein kinase and blocks the cAMP-induced dissociation of regulatory and catalytic subunits of the enzyme whereby the enzyme is inactivated. The mechanism by which hemin prevents the formation of the inhibitor and maintains protein synthesis in reticulocyte lysates is thus explained.

MeSH Terms
Animals Binding, Competitive Cyclic AMP/pharmacology Enzyme Activation/drug effects Heme/analogs & derivatives Hemin/pharmacology Kinetics Protamine Kinase/blood Protein Biosynthesis/drug effects Rabbits Reticulocytes/metabolism
Chemicals
Heme Hemin Cyclic AMP Protamine Kinase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Datta A
de Haro C
Sierra J M
Ochoa S
References (43)
43 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.8.3326
Published
1977-08-00
Pages
3326-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431549
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.8.3326 PMC Full Text View journal details
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