Home LiteratureArticle Details
PMID: 4372990 Published · ppublish English Journal Article

Anti-anabolic effects of adenosine 3':5'-cyclic monophosphate. Inhibition of protein synthesis.

The Biochemical journal ·Vol. 138 ·No. 3 ·1974-03-00 ·Pages 335-40

Sellers A, Bloxham DP, Munday KA, Akhtar M

Abstract

1. Evidence is presented that cyclic AMP inhibits the incorporation of l-[4,5-(3)H]leucine into protein in a cell-free system from rat liver. This inhibition occurs after aminoacyl-tRNA formation. 2. Microsomal fractions, isolated after the incubation of postmitochondrial supernatant with cyclic AMP and ATP, show a diminished ability to synthesize protein. Both cyclic AMP and ATP are required for this effect. 3. A possible physiological role for the anti-anabolic action of cyclic AMP is discussed in terms of the control of gluconeogenesis.

MeSH Terms
Adenosine Triphosphate/metabolism Animals Cell-Free System Centrifugation Circadian Rhythm Cyclic AMP/pharmacology Gluconeogenesis/drug effects In Vitro Techniques Leucine/metabolism Liver/drug effects,metabolism Male Microsomes, Liver/metabolism Phosphoenolpyruvate Protein Biosynthesis RNA, Transfer/biosynthesis Rats Transfer RNA Aminoacylation Tritium Ultracentrifugation
Chemicals
Tritium Phosphoenolpyruvate Adenosine Triphosphate RNA, Transfer Cyclic AMP Leucine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sellers A
Bloxham D P
Munday K A
Akhtar M
References (22)
22 references, click to expand
  1. Studies on the control of cholesterol biosynthesis: the adenosine 3':5'-cyclic monophosphate-dependent accumulation of a steroid carboxylic acid.
    Biochem J. 1971 Jun;123(2):275-8 PMID: 4331371
  2. Studies on carbohydrate metabolism in rat liver slices. IV. Biochemical sequence of events after insulin administration.
    J Biol Chem. 1955 Mar;213(1):135-46 PMID: 14353913
  3. The hormonal control of hepatic gluconeogenesis.
    Recent Prog Horm Res. 1970;26:411-61 PMID: 4319350
  4. Evidence for regulatin of cholesterol and fatty acid synthesis in liver by cyclic adenosine 3',5'-monophosphate.
    J Biol Chem. 1972 Aug 10;247(15):4914-5 PMID: 4340705
  5. The effect of chemical agents on the turnover of the bound phosphate associated with the sodium-and-potassium ion-stimulated adenosine triphosphatase in ox brain microsomes.
    Biochem J. 1970 Nov;120(1):1-13 PMID: 4250238
  6. Respiratory granules of heart muscle.
    Biochem J. 1953 Mar;53(4):547-56 PMID: 13032108
  7. Role of adenosine 3',5'-monophosphate in the effects of insulin and anti-insulin serum on liver metabolism.
    J Biol Chem. 1968 Mar 10;243(5):1031-8 PMID: 5640968
  8. Studies on the inhibition of amino acid incorporation into protein by isolated rat liver ribosomes by protein kinase.
    Biochem Biophys Res Commun. 1972 Mar 10;46(5):1881-6 PMID: 4335570
  9. Effects of glucagon on amino acid transport and utilization in the perfused rat liver.
    J Biol Chem. 1969 Oct 25;244(20):5724-8 PMID: 4310605
  10. Control of gluconeogenesis from amino acids in the perfused rat liver.
    J Biol Chem. 1969 Oct 25;244(20):5713-23 PMID: 4310604
  11. Differential effects of glucocorticoids and adenosine 3',5'-monophosphate on hepatic enzyme synthesis.
    J Biol Chem. 1971 Jan 10;246(1):217-23 PMID: 4322440
  12. Modulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase activity with cAMP and wth protein fractions of rat liver cytosol.
    Biochem Biophys Res Commun. 1973 Oct 15;54(4):1362-9 PMID: 4356818
  13. Incorporation of C14-amino acids into glutathione and protein fractions of normal and diabetic rat tissues.
    J Biol Chem. 1953 Jan;200(1):99-109 PMID: 13034763
  14. Diurnal fluctuation and -adrenergic elevation of cyclic AMP in mouse epidermis in vivo.
    Nat New Biol. 1972 Dec 6;240(101):178-9 PMID: 4343948
  15. Control of protein synthesis in mammalian tissues through changes in ribosome activity.
    J Biol Chem. 1971 Jan 25;246(2):436-46 PMID: 5542013
  16. Phosphorylation of rat liver ribosomal subunits: partial purification of two cyclic AMP activated protein kinases.
    Biochem Biophys Res Commun. 1971 Jun 4;43(5):1001-9 PMID: 4327953
  17. Phosphorylation of some rat liver ribosomal proteins and its activation by cyclic AMP.
    FEBS Lett. 1970 Sep 24;10(2):105-108 PMID: 11945368
  18. Biosynthesis of labeled fatty acids and cholesterol in experimental diabetes.
    J Biol Chem. 1950 Dec;187(2):589-96 PMID: 14803440
  19. Regulation of fatty acid synthesis in isolated hepatocytes prepared from the livers of neonatal chicks.
    J Biol Chem. 1973 Mar 25;248(6):1924-31 PMID: 4347857
  20. Studies on the mechanism and regulation of C-4 demethylation in cholesterol biosynthesis. The role of adenosine 3':5'-cyclic monophosphate.
    Biochem J. 1971 Nov;125(2):625-34 PMID: 4401381
  21. THE CROONIAN LECTURE, 1963. GLUCONEOGENESIS.
    Proc R Soc Lond B Biol Sci. 1964 Mar 17;159:545-64 PMID: 14130854
  22. Citrate and the conversion of carbohydrate into fat.
    Biochem Soc Symp. 1968;27:61-86 PMID: 5761101
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1380335
Published
1974-03-00
Pages
335-40
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1166218
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1380335 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2