Frog oocytes synthesize and completely process the precursor polypeptide to virion structural proteins after microinjection of avian myeloblastosis virus RNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 8 ·1977-08-00 ·Pages 3230-4

Ghysdael J, Hubert E, Trávnícek M, Bolognesi DP, Burny A, Cleuter Y, Huez G, Kettmann R, Marbaix G, Portetelle D, Chantrenne H

Abstract

After microinjection of Xenopus laevis oocytes with RNA from avian myeloblastosis virus, viral structural proteins p27, p19, p15, and p12 are formed by a sequence of posttranslational cleavages of a high-molecular-weight precursor polypeptide. The 60-70S RNA aggregate or its 30-40S RNA subunits obtained by heat or formamide treatment possess the same ability to serve as template in X. laevis oocytes. The processing pattern of virus-specific precursor polypeptides is the same in X. laevis oocytes as in chick embryo fibroblasts infected with avian myeloblastosis virus, but the processing takes place at a much slower rate.

MeSH Terms

Animals Avian Leukosis Virus/metabolism Avian Myeloblastosis Virus/metabolism Chick Embryo Female Fibroblasts/metabolism Microinjections Molecular Weight Oocytes/metabolism Ovum/metabolism Peptide Biosynthesis Precipitin Tests Protein Biosynthesis RNA, Viral/metabolism Viral Proteins/biosynthesis Xenopus

Chemicals

RNA, Viral Viral Proteins

Authors & Affiliations

11 authors, click to expand affiliations / ORCID

Ghysdael J

Hubert E

Trávnícek M

Bolognesi D P

Burny A

Cleuter Y

Huez G

Kettmann R

Marbaix G

Portetelle D

Chantrenne H

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24 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.74.8.3230

Published

1977-08-00

Pages

3230-4

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC431509

Subset

External Links

PubMed source DOI: 10.1073/pnas.74.8.3230 PMC Full Text View journal details

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Frog oocytes synthesize and completely process the precursor polypeptide to virion structural proteins after microinjection of avian myeloblastosis virus RNA.

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