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PMID: 19805315
Published · ppublish
English
Journal Article
Research Support, Non-U.S. Gov't
Community proteogenomics reveals insights into the physiology of phyllosphere bacteria.
Proceedings of the National Academy of Sciences of the United States of America
·Vol. 106
·No. 38
·2009-09-22
·Pages 16428-33
Abstract
Aerial plant surfaces represent the largest biological interface on Earth and provide essential services as sites of carbon dioxide fixation, molecular oxygen release, and primary biomass production. Rather than existing as axenic organisms, plants are colonized by microorganisms that affect both their health and growth. To gain insight into the physiology of phyllosphere bacteria under in situ conditions, we performed a culture-independent analysis of the microbiota associated with leaves of soybean, clover, and Arabidopsis thaliana plants using a metaproteogenomic approach. We found a high consistency of the communities on the 3 different plant species, both with respect to the predominant community members (including the alphaproteobacterial genera Sphingomonas and Methylo bacterium) and with respect to their proteomes. Observed known proteins of Methylobacterium were to a large extent related to the ability of these bacteria to use methanol as a source of carbon and energy. A remarkably high expression of various TonB-dependent receptors was observed for Sphingomonas. Because these outer membrane proteins are involved in transport processes of various carbohydrates, a particularly large substrate utilization pattern for Sphingomonads can be assumed to occur in the phyllosphere. These adaptations at the genus level can be expected to contribute to the success and coexistence of these 2 taxa on plant leaves. We anticipate that our results will form the basis for the identification of unique traits of phyllosphere bacteria, and for uncovering previously unrecorded mechanisms of bacteria-plant and bacteria-bacteria relationships.
MeSH Terms
Bacteria/classification,genetics,metabolism
Bacterial Physiological Phenomena
Bacterial Proteins/analysis
DNA, Bacterial/chemistry,genetics
Ecosystem
Electrophoresis, Polyacrylamide Gel
Genomics/methods
Molecular Sequence Data
Phylogeny
Plants/microbiology
Proteomics/methods
RNA, Ribosomal, 16S/genetics
Sequence Analysis, DNA
Spectrometry, Mass, Electrospray Ionization
Chemicals
Bacterial Proteins
DNA, Bacterial
RNA, Ribosomal, 16S
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
1091-6490
Published
2009-09-22
Epub
2009-00-04
Pages
16428-33
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC2738620
Subset
IM
Databases
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