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PMID: 1973599 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The mesangium in anti-Thy-1 nephritis. Influx of macrophages, mesangial cell hypercellularity, and macromolecular accumulation.

The American journal of pathology ·Vol. 137 ·No. 1 ·1990-07-00 ·Pages 215-23

Bagchus WM, Jeunink MF, Elema JD

Abstract

Mesangial pathology is a hallmark of focal and segmental glomerulosclerosis (FGS). In an immunologically mediated mesangial cell (MC) injury model, we analyzed the relationship between mesangial hypercellularity, increased macromolecular uptake by the mesangium, and long-term pathologic sequelae. A single injection of monoclonal anti-Thy-1 (AT) antibody induces MC apoptosis, extensive mesangiolysis, proteinuria, MC proliferation, and hypercellularity. Immunohistologic analysis indicated influx of ED 1-positive macrophages after 24 hours, which gradually subsiding thereafter. At day 12, hypercellularity was due to smooth musclelike MCs, and macrophagelike MCs were absent. Injection of iron dextran in nephritic rats indicated that mesangial uptake of iron correlated with mesangial hypercellularity, but was independent of proteinuria. Long-term studies showed no difference after 19 weeks in FGS between nephritic and control rats. In conclusion, although mesangiolysis is accompanied by influx of macrophages, a phase of smooth musclelike MC proliferation and increased macromolecular uptake, these pathologic events do not result in chronic mesangial pathology and FGS.

MeSH Terms
Animals Antigens, Surface/immunology Glomerular Mesangium/pathology Glomerulosclerosis, Focal Segmental/pathology Iron/pharmacokinetics Macrophages/metabolism,pathology,ultrastructure Male Mice Mice, Inbred BALB C Muscle, Smooth, Vascular/metabolism,pathology Nephritis/pathology Rats Rats, Inbred Strains Thy-1 Antigens
Chemicals
Antigens, Surface Thy-1 Antigens Iron
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bagchus W M
Department of Pathology, University of Groningen, The Netherlands.
Jeunink M F
Elema J D
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
Published
1990-07-00
Pages
215-23
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1877695
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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