The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognized by monoclonal antibodies ED1, ED2 and ED3.

Immunology ·Vol. 54 ·No. 3 ·1985-03-00 ·Pages 589-99

Dijkstra CD, Döpp EA, Joling P, Kraal G

Abstract

In the present study, a set of three monoclonal antibodies is described, each of which recognizes cells of the monocyte-macrophage lineage in the rat. The tissue distribution, in particular in lymphoid organs, of each of the three monoclonals is determined by immunoenzyme histochemistry on cryostat sections, as well as on cell suspensions. Results show that ED1 recognizes a cytoplasmic antigen in monocytes and in most macrophages, free and fixed. ED2 and ED3 recognize membrane antigens of tissue macrophages, discriminating between distinct subpopulations of macrophages, each with a characteristic localization in the compartments of lymphoid organs. No other cell types except cells of the mononuclear phagocyte system are positive for any of the three monoclonals. Possible relations between the macrophages recognized by this set of monoclonals and dendritic cells are discussed.

MeSH Terms

Animals Antibodies, Monoclonal Antibody Specificity Antigens, Surface/immunology Bone Marrow/immunology Cells, Cultured Cytoplasm/immunology Immunoenzyme Techniques Lymphoid Tissue/immunology Macrophages/immunology Mice Mice, Inbred BALB C Phagocytes/immunology Rats Rats, Inbred Strains

Chemicals

Antibodies, Monoclonal Antigens, Surface

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Dijkstra C D

Döpp E A

Joling P

Kraal G

References (27)

27 references, click to expand

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Article Info

Journal

Immunology

Abbr.

Immunology

ISSN

0019-2805

Published

1985-03-00

Pages

589-99

Language

English

Region

England

NLM ID

0374672

PMCID

PMC1453512

Subset

External Links

PubMed source PMC Full Text View journal details

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The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognized by monoclonal antibodies ED1, ED2 and ED3.

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