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PMID: 6574493 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Staining of Langerhans cells with monoclonal antibodies to macrophages and lymphoid cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 11 ·1983-06-00 ·Pages 3448-51

Haines KA, Flotte TJ, Springer TA, Gigli I, Thorbecke GJ

Abstract

Langerhans cells are Ia-bearing antigen-presenting cells in the epidermis that share many functions with macrophages. We have used monoclonal antibodies to the macrophage antigens, Mac-2 and-3, Ia antigen, Fc fragment receptor and the common leukocyte antigen CLA to compare the cell surface antigens of these cells with those of interdigitating and follicular dendritic cells and of macrophages in lymphoid tissues. Immunoperoxidase staining was carried out with epidermal sheets from BALB/c mice and epidermal cell suspensions enriched for Langerhans cells by Fc rosetting. Langerhans cells stained for all of these antigens. Comparison with the staining properties of other dendritic cells and macrophages, in combination with previous observations, indicates a close relationship of Langerhans cells to the interdigitating cells of lymphoid tissues.

MeSH Terms
Animals Antibodies, Monoclonal Antigens, Surface/analysis Histocompatibility Antigens Class II/analysis Langerhans Cells/immunology Lymphocytes/immunology Macrophages/immunology Mice Mice, Inbred BALB C
Chemicals
Antibodies, Monoclonal Antigens, Surface Histocompatibility Antigens Class II
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Haines K A
Flotte T J
Springer T A
Gigli I
Thorbecke G J
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45 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.11.3448
Published
1983-06-00
Pages
3448-51
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC394061
Subset
IM
Grants
NIAID NIH HHS · AI-03076 · United States
NIAID NIH HHS · AI-17365 · United States
NCI NIH HHS · CA-31799 · United States
External Links
PubMed source DOI: 10.1073/pnas.80.11.3448 PMC Full Text View journal details
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