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PMID: 150949 Published · ppublish English Journal Article

Dendritic cells in the rat spleen follicles. A combined immuno- and enzyme histochemical study.

Cell and tissue research ·Vol. 190 ·No. 1 ·1978-06-26 ·Pages 79-87

Eikelenboom P

Abstract

Follicles of peripheral lymphoid organs (rat) contain a type of non-lymphoid cell which is capable of arresting antigen-antibody complexes at the cell surface. These so-called dendritic cells can be visualized in immunized rats by staining antigen-antibody complexes with immunohistoperoxidase techniques. The present study concerns a classification of these cells and comparison with known non-lymphoid cell types such as macrophages, marginal metallophils and tingible body macrophages in the rat spleen follicles. Immunoenzyme histochemical and (enzyme) histochemical techniques have been combined in the same tissue sections to correlate the functional capacity of binding immune complexes with morphological characteristics or phagocytic capacity. Dendritic cells show silver affinity but do not demonstrate a characteristic pattern of hydrolytic enzymes or phagocytosis.

MeSH Terms
Acid Phosphatase/metabolism Adenosine Triphosphatases/metabolism Alkaline Phosphatase/metabolism Animals Esterases/metabolism Histocytochemistry Immunoenzyme Techniques Macrophages/immunology Nucleotidases/metabolism Phagocytosis Rats Spleen/cytology,enzymology,immunology
Chemicals
Esterases Nucleotidases Alkaline Phosphatase Acid Phosphatase Adenosine Triphosphatases
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Eikelenboom P
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21 references, click to expand
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Article Info
Journal
Cell and tissue research
Abbr.
Cell Tissue Res
ISSN
0302-766X
DOI
10.1007/BF00210038
Published
1978-06-26
Pages
79-87
Language
English
Region
Germany
NLM ID
0417625
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00210038 View journal details
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