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PMID: 1970565 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Rhizobium meliloti adenylate cyclase is related to eucaryotic adenylate and guanylate cyclases.

Journal of bacteriology ·Vol. 172 ·No. 5 ·1990-05-00 ·Pages 2614-21

Beuve A, Boesten B, Crasnier M, Danchin A, O'Gara F

Abstract

A gene from Rhizobium meliloti coding for an adenylate cyclase was sequenced, and the deduced protein sequence was compared with those of other known adenylate cyclases. No similarity could be detected with the procaryotic counterparts. However, striking similarity was found with the catalytic region of Saccharomyces cerevisiae adenylate cyclase, the cytoplasmic domains of bovine adenylate cyclase, and two mammalian guanylate cyclases. The gene was fused to the enteric beta-galactosidase, and the chimeric protein was purified by affinity chromatography. This fusion protein was found to direct the synthesis of cyclic AMP in vitro. This activity was strongly inhibited by the presence of GTP, but no cyclic GMP synthesis could be detected in conditions permitting cyclic AMP synthesis.

MeSH Terms
Adenylyl Cyclases/genetics,metabolism Amino Acid Sequence Animals Base Sequence Cattle Chromosome Deletion Cloning, Molecular Escherichia coli/genetics Genes Genes, Bacterial Genotype Guanylate Cyclase/genetics Molecular Sequence Data Plasmids Rats Restriction Mapping Rhizobium/enzymology,genetics Saccharomyces cerevisiae/enzymology,genetics Sequence Homology, Nucleic Acid beta-Galactosidase/genetics,metabolism
Chemicals
beta-Galactosidase Adenylyl Cyclases Guanylate Cyclase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Beuve A
Institut Pasteur, Paris, France.
Boesten B
Crasnier M
Danchin A
O'Gara F
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34 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.5.2614-2621.1990
Published
1990-05-00
Pages
2614-21
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC208905
Subset
IM
Databases
GENBANK
M35096
External Links
PubMed source DOI: 10.1128/jb.172.5.2614-2621.1990 PMC Full Text View journal details
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