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PMID: 1970242 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Distribution of hepatic glutaminase activity and mRNA in perivenous and periportal rat hepatocytes.

The Biochemical journal ·Vol. 267 ·No. 1 ·1990-04-01 ·Pages 265-7

Watford M, Smith EM

Abstract

Perivenous and periportal hepatocytes were isolated by the digitonin/collagenase perfusion technique. The specific activity of phosphate-activated glutaminase was 2.33-fold higher in periportal cells than in perivenous cells. Similarly, the relative abundance of glutaminase mRNA was 2.6-fold higher in samples from periportal cells. The distribution of glutaminase activity and mRNA was compared with those for glutamine synthetase (predominantly perivenous) and phosphoenolpyruvate carboxykinase (predominantly periportal). The results suggest that phosphate-activated glutaminase is predominantly expressed in the periportal zone of the liver acinus.

MeSH Terms
Animals Glutamate-Ammonia Ligase/genetics,metabolism Glutaminase/genetics,metabolism Hepatic Veins Liver/blood supply,enzymology Male Nucleic Acid Hybridization Phosphates/pharmacology Phosphoenolpyruvate Carboxykinase (GTP)/genetics,metabolism Portal Vein RNA, Messenger/metabolism Rats Rats, Inbred Strains Tissue Distribution
Chemicals
Phosphates RNA, Messenger Glutaminase Phosphoenolpyruvate Carboxykinase (GTP) Glutamate-Ammonia Ligase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Watford M
Division of Nutritional Sciences, Cornell University, Ithaca, NY 14853.
Smith E M
References (19)
19 references, click to expand
  1. Isolation and characterization of the gene coding for cytosolic phosphoenolpyruvate carboxykinase (GTP) from the rat.
    Proc Natl Acad Sci U S A. 1983 Jun;80(12):3656-60 PMID: 6304730
  2. Functional specialization of different hepatocyte populations.
    Physiol Rev. 1989 Jul;69(3):708-64 PMID: 2664826
  3. Immunohistochemical localization of carbamoyl-phosphate synthetase (ammonia) in adult rat liver; evidence for a heterogeneous distribution.
    J Histochem Cytochem. 1984 Jun;32(6):557-64 PMID: 6373912
  4. Gluconeogenesis in periportal and perivenous hepatocytes of rat liver, isolated by a new high-yield digitonin/collagenase perfusion technique.
    Biochem J. 1985 Jul 1;229(1):221-6 PMID: 2994630
  5. Selection of a rat glutamine synthetase cDNA clone.
    Biochem Biophys Res Commun. 1986 Jan 14;134(1):146-51 PMID: 2418824
  6. Rat hepatic glutaminase: purification and immunochemical characterization.
    Arch Biochem Biophys. 1988 Feb 1;260(2):740-51 PMID: 3277537
  7. Binding of adenosine triphosphate and adenosine diphosphate by glutamine synthetase.
    Biochemistry. 1966 Mar;5(3):872-9 PMID: 5911299
  8. Phosphoenolpyruvate carboxykinase and pyruvate carboxylase in developing rat liver.
    Biochem J. 1967 Sep;104(3):866-71 PMID: 6049928
  9. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  10. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7 PMID: 291033
  11. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
  12. Hepatocyte heterogeneity in glutamine and ammonia metabolism and the role of an intercellular glutamine cycle during ureogenesis in perfused rat liver.
    Eur J Biochem. 1983 Jun 15;133(2):269-75 PMID: 6852039
  13. Zonation of glycogen and glucose syntheses, but not glycolysis, in rat liver.
    Biochem J. 1988 Oct 1;255(1):99-104 PMID: 3143359
  14. Heterogeneous expression of glutamine synthetase mRNA in rat liver parenchyma revealed by in situ hybridization and Northern blot analysis of RNA from periportal and perivenous hepatocytes.
    FEBS Lett. 1988 Dec 5;241(1-2):89-93 PMID: 2904380
  15. Positional and developmental regulation of glutamine synthetase expression in mouse liver.
    Mol Cell Biol. 1988 Nov;8(11):4966-71 PMID: 2905422
  16. Localization of ammonia-metabolizing enzymes in human liver: ontogenesis of heterogeneity.
    Hepatology. 1989 Mar;9(3):367-72 PMID: 2563984
  17. Predominant localization of phosphoenolpyruvate carboxykinase mRNA in the periportal zone of rat liver parenchyma demonstrated by in situ hybridization.
    FEBS Lett. 1989 May 8;248(1-2):188-94 PMID: 2721676
  18. The gamma-glutamyltransferase/glutamine synthetase activity ratio. A powerful marker for the acinar origin of hepatocytes.
    J Hepatol. 1989 May;8(3):338-43 PMID: 2567297
  19. Heterogeneous distribution of glutamine synthetase among rat liver parenchymal cells in situ and in primary culture.
    EMBO J. 1983;2(4):567-70 PMID: 6138251
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2670265
Published
1990-04-01
Pages
265-7
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1131276
Subset
IM
Grants
NIDDK NIH HHS · DK-37301 · United States
External Links
PubMed source DOI: 10.1042/bj2670265 PMC Full Text View journal details
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